Back to Search
Start Over
Role of glutathione in the growth of Bradyrhizobium sp. (peanut microsymbiont) under different environmental stresses and in symbiosis with the host plant
- Source :
- Canadian Journal of Microbiology. 52:609-616
- Publication Year :
- 2006
- Publisher :
- Canadian Science Publishing, 2006.
-
Abstract
- Glutathione (GSH) plays an important role in the defence of microorganisms and plants against different environmental stresses. To determine the role of GSH under different stresses, such as acid pH, saline shock, and oxidative shock, a GSH-deficient mutant (Bradyrhizobium sp. 6144-S7Z) was obtained by disruption of the gshA gene, which encodes the enzyme γ-glutamylcysteine synthetase. Growth of the mutant strain was significantly reduced in liquid minimal saline medium, and the GSH content was very low, about 4% of the wild-type level. The defect, caused by disruption of the gshA gene in the growth of mutant strain, cannot be reversed by the addition of GSH (up to 100 µmol/L) to the liquid minimal saline medium, and the endogenous GSH level was approximately the same as that observed without the addition of GSH. In contrast, the wild-type strain increased the GSH content under these conditions. However, the growth of the mutant strain in a rich medium (yeast extract – mannitol) increased, suggesting that at least some but not all of the functions of GSH could be provided by peptides and (or) amino acids. The symbiotic properties of the mutant were similar to those found in the wild-type strain, indicating that the mutation does not affect the ability of the mutant to form effective nodules.Key words: glutathione, γ-glutamylcysteine synthetase, Bradyrhizobium sp., peanut.
- Subjects :
- Rhizobiaceae
Arachis
Glutamate-Cysteine Ligase
Immunology
Mutant
Oxidative phosphorylation
Sodium Chloride
Plant Roots
Applied Microbiology and Biotechnology
Microbiology
Bradyrhizobium
chemistry.chemical_compound
Genetics
medicine
Yeast extract
Symbiosis
Molecular Biology
biology
Strain (chemistry)
General Medicine
Glutathione
Hydrogen-Ion Concentration
biology.organism_classification
Oxidative Stress
Biochemistry
chemistry
Mannitol
medicine.drug
Subjects
Details
- ISSN :
- 14803275 and 00084166
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- Canadian Journal of Microbiology
- Accession number :
- edsair.doi.dedup.....86729a6c805c7e875ef20f17d2fdcfb5
- Full Text :
- https://doi.org/10.1139/w06-007