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Isolation and Molecular Typing of Leishmania infantum from Phlebotomus perfiliewi in a Re-Emerging Focus of Leishmaniasis, Northeastern Italy
- Source :
- Microorganisms, Volume 7, Issue 12
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Visceral leishmaniasis (VL) caused by Leishmania (L.) infantum is a public health threat in the Emilia-Romagna region, northeastern Italy, but its epidemiology has not been fully elucidated in this area. The objective of this study was to characterize Leishmania infection in sand flies collected in a re-emerging focus of VL in the Bologna province. During the summer of 2016, 6114 sand flies were collected, identified, and tested for Leishmania detection. Of the identified sand flies, 96.5% were Phlebotomus (P.) perfiliewi and 3.5% were P. perniciosus. Detected parasites were characterized by biomolecular methods (multilocus microsatellite typing and characterization of repetitive region on chromosome 31), and quantified by real-time PCR. The prevalence of Leishmania infection in individually-tested P. perfiliewi sand flies varied from 6% to 10% with an increasing trend during the season. Promastigotes of L. infantum were isolated by dissection in one P. perfiliewi female<br />the isolated strain (Lein-pw) were closely related to Leishmania parasites from VL cases in northeastern Italy, but differed from strains isolated in dogs from the same area. Our findings strongly support the vector status of P. perfiliewi for human VL in the study area.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Veterinary medicine
030231 tropical medicine
Prevalence
Biology
Microbiology
Leishmania infantum
Quantitative PCR
parasite load
03 medical and health sciences
0302 clinical medicine
Virology
parasitic diseases
medicine
visceral leishmaniasis
Typing
Phlebotomus
fungi
molecular typing
Leishmaniasis
medicine.disease
Leishmania
biology.organism_classification
Phlebotomus perniciosu
030104 developmental biology
Visceral leishmaniasis
Phlebotomus perfiliewi
Vector (epidemiology)
Phlebotomus perniciosus
quantitative PCR
Subjects
Details
- ISSN :
- 20762607
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Microorganisms
- Accession number :
- edsair.doi.dedup.....866b9c23318f4cc2245c199f641d125c