Back to Search
Start Over
Murine Monoclonal Antibodies for Antigenic Discrimination of HIV-1 Envelope Proteins
- Publication Year :
- 2016
- Publisher :
- Mary Ann Liebert, Inc., 2016.
-
Abstract
- In the influenza virus field, antibody reagents from research animals have been instrumental in the characterization of antigenically distinct hemagglutinin and neuraminidase membrane molecules. These small animal reagents continue to support the selection of components for inclusion in human influenza virus vaccines. Other cocktail vaccines against variant pathogens (e.g., polio virus, pneumococcus) are similarly designed to represent variant antigens, as defined by antibody reactivity patterns. However, a vaccine cocktail comprising diverse viral membrane antigens defined in this way has not yet been advanced to a clinical efficacy study in the HIV-1 field. In this study, we describe the preparation of mouse antibodies specific for HIV-1 gp140 or gp120 envelope molecules. Our experiments generated renewable reagents able to discriminate HIV-1 envelopes from one another. Monoclonals yielded more precise discriminatory capacity against their respective immunogens than did a small panel of polyclonal human sera derived from recently HIV-1-infected patients. Perhaps these and other antibody reagents will ultimately support high-throughput cartography studies with which antigenically-distinct envelope immunogens may be formulated into a successful HIV-1 envelope cocktail vaccine.
- Subjects :
- 0301 basic medicine
Adult
Adolescent
medicine.drug_class
Immunology
Hemagglutinin (influenza)
HIV Infections
HIV Antibodies
HIV Envelope Protein gp120
Monoclonal antibody
Virus
03 medical and health sciences
Mice
Young Adult
Antigen
Virology
medicine
Animals
Humans
AIDS Vaccines
biology
business.industry
Brief Report
env Gene Products, Human Immunodeficiency Virus
virus diseases
Antibodies, Monoclonal
Viral membrane
Mice, Inbred C57BL
030104 developmental biology
biology.protein
HIV-1
Molecular Medicine
Immunization
Antibody
business
Neuraminidase
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....86635ca3a101980f65d764d7952ba18e