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Calu-3 epithelial cells exhibit different immune and epithelial barrier responses from freshly isolated primary nasal epithelial cells in vitro
- Source :
- Clinical and translational allergy, 8(1):02258. BioMed Central, Clinical and Translational Allergy, Vol 8, Iss 1, Pp 1-4 (2018), Clinical and Translational Allergy
- Publication Year :
- 2018
-
Abstract
- Epithelial cell lines are often used to evaluate the effect of exogenous/endogenous stimuli on epithelial barrier function and innate immune responses in allergic airway diseases, without clear view on differences between epithelial cell lines and primary nasal epithelial cell responses. In this observational study, we compared the response of Calu-3 and primary nasal epithelial cells to two relevant exogenous stimuli: i.e. Staphylococcus aureus enterotoxin B (SEB) and house dust mite (HDM). Stimulation of Calu-3 cells with SEB decreased epithelial integrity in a dose dependent manner, which was associated with a significant increase in IL-6 and IL-8 production. In contrast, no alteration in barrier integrity or IL-6 and IL-8 production was seen when primary nasal epithelial cells were stimulated with SEB. HDM extract altered the integrity of primary nasal epithelial cells, but not of Calu-3 epithelial cells. Increased IL-8 production was seen after stimulation with HDM in primary nasal epithelial cells and not in Calu-3 epithelial cells. In conclusion, immune and barrier function differ between different epithelial cell types studied. As a consequence, care must be taken when interpreting data using different epithelial cell types. Electronic supplementary material The online version of this article (10.1186/s13601-018-0225-8) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Pulmonary and Respiratory Medicine
Allergy
Immunology
Calu-3 epithelial cell line
Endogeny
Stimulation
Trans-epithelial electrical resistance
03 medical and health sciences
Immune system
AUREUS ENTEROTOXIN-B
HOUSE-DUST MITE
Primary nasal epithelial cells
Immunology and Allergy
Medicine
Letter to the Editor
Barrier function
House dust mite
Science & Technology
Innate immune system
biology
business.industry
ALLERGIC SENSITIZATION
RC581-607
biology.organism_classification
DYSFUNCTION
In vitro
Epithelium
Cell biology
030104 developmental biology
medicine.anatomical_structure
ASTHMA
House dust mite extract
Staphylococcus aureus enterotoxin B
Immunologic diseases. Allergy
business
Life Sciences & Biomedicine
Subjects
Details
- Language :
- English
- ISSN :
- 20457022
- Database :
- OpenAIRE
- Journal :
- Clinical and translational allergy, 8(1):02258. BioMed Central, Clinical and Translational Allergy, Vol 8, Iss 1, Pp 1-4 (2018), Clinical and Translational Allergy
- Accession number :
- edsair.doi.dedup.....8642b8ad7734cf6f4148ce5b2c15fdff