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Use of IP-10 detection in dried plasma spots for latent tuberculosis infection diagnosis in contacts via mail
- Source :
- Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019), Scientific Reports, r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol, instname, Scientia, Dipòsit Digital de Documents de la UAB, Universitat Autònoma de Barcelona
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group, 2019.
-
Abstract
- The aim of this study was to test the use of IP-10 detection in dried plasma from contact studies individuals (contacts of smear positive patients), by comparing it with IP-10 and IFN-γ detection in direct plasma, to establish IP-10 detection in DPS as a useful assay for LTBI diagnosis. Whole blood samples were collected from 80 subjects: 12 with active tuberculosis (TB), and 68 from contact studies. The amount of IFN-γ produced by sensitized T cells was determined in direct plasma by QuantiFERON Gold In-Tube test. IP-10 levels were determined in direct and dried plasma by an in-house ELISA. For dried plasma IP-10 determination, two 25 µl plasma drops were dried in Whatman903 filter paper and sent by mail to the laboratory. Regarding TB patients, 100.0%, 91.7% and 75.0% were positive for IFN-γ detection and IP-10 detection in direct and dried plasma, respectively. In contacts, 69.1%, 60.3% and 48.5% had positive results after IFN-γ and IP-10 in direct and dried plasma, respectively. The agreement among in vitro tests was substantial and IP-10 levels in direct and dried plasma were strongly correlated (r = 0.897). In conclusion, IP-10 detection in dried plasma is a simple and safe method that would help improve LTBI management.
- Subjects :
- 0301 basic medicine
Adult
Male
Tuberculosis
aminoácidos, péptidos y proteínas::péptidos::péptidos y proteínas de señalización intercelular::citocinas::quimiocinas::quimiocinas CXC::quimiocina CXCL10 [COMPUESTOS QUÍMICOS Y DROGAS]
Otros calificadores::/diagnóstico [Otros calificadores]
Tuberculosi - Diagnòstic
lcsh:Medicine
Enzyme-Linked Immunosorbent Assay
Sensitivity and Specificity
Article
QuantiFERON
03 medical and health sciences
0302 clinical medicine
Latent Tuberculosis
Other subheadings::/diagnosis [Other subheadings]
medicine
Humans
Postal Service
Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Actinomycetales Infections::Mycobacterium Infections::Tuberculosis::Latent Tuberculosis [DISEASES]
lcsh:Science
Whole blood
Multidisciplinary
Chromatography
Latent tuberculosis
Filter paper
Spots
business.industry
infecciones bacterianas y micosis::infecciones bacterianas::infecciones por bacterias grampositivas::infecciones por Actinomycetales::micobacteriosis::tuberculosis::tuberculosis latente [ENFERMEDADES]
técnicas de investigación::métodos epidemiológicos::rastreo de contactos [TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS]
lcsh:R
Infection diagnosis
Plasma
medicine.disease
Quimiocines
Chemokine CXCL10
030104 developmental biology
Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Cytokines::Chemokines::Chemokines, CXC::Chemokine CXCL10 [CHEMICALS AND DRUGS]
ROC Curve
Cytokines
Female
lcsh:Q
Dried Blood Spot Testing
Investigative Techniques::Epidemiologic Methods::Contact Tracing [ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES AND EQUIPMENT]
Contact Tracing
business
030217 neurology & neurosurgery
Interferon-gamma Release Tests
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....86318b8174c9fa8209ffaa6d20f49d08