Back to Search
Start Over
Involvement of AMP-activated protein kinase in the protective effectof melatonin against renal ischemia reperfusion injury
- Source :
- Volume: 40, Issue: 4 837-844, Turkish Journal of Biology
- Publication Year :
- 2016
- Publisher :
- The Scientific and Technological Research Council of Turkey (TUBITAK-ULAKBIM) - DIGITAL COMMONS JOURNALS, 2016.
-
Abstract
- We investigated the implication of AMP-activated protein kinase (AMPK) in melatonin-induced protection in a renal ischemia/reperfusion (I/R) model. Animals were divided into four groups: rats of the sham group were not subjected to renal I/R. Rats in the I/R group received the vehicle 30 min before renal ischemia for 1 h followed by reperfusion for 6 h. Rats in the Mel+I/R group received melatonin (10 mg/kg) 30 min before the onset of I/R. Rats in the Mel+I/R+araA group received araA (AMPK inhibitor, 100 μg/kg per min for 10 min) just before melatonin administration. The results showed that melatonin treatment induced a significant improvement in renal function, morphology, and antioxidant status, as well as Akt pathway activation. It also decreased cytolysis and endoplasmic reticulum stress. Inhibition of AMPK by araA administration completely abolished the effects of melatonin in regard to the above parameters except for Akt and some of its downstream target molecules. These results demonstrate that the effects of melatonin on renal I/R are, for the most part, linked to AMPK activation, with the exception of the Akt pathway, which seems to be independent of AMPK.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Physiology
Biology
Microbiology
Melatonin
03 medical and health sciences
AMP-activated protein kinase
Internal medicine
Genetics
medicine
Protein kinase A
Molecular Biology
Protein kinase B
PI3K/AKT/mTOR pathway
Kidney
Renal ischemia
Ischemia/reperfusion,kidney,melatonin,AMP-activated protein kinase,oxidative stress,Akt
AMPK
Cell Biology
030104 developmental biology
medicine.anatomical_structure
Endocrinology
biology.protein
General Agricultural and Biological Sciences
medicine.drug
Subjects
Details
- ISSN :
- 13036092 and 13000152
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- TURKISH JOURNAL OF BIOLOGY
- Accession number :
- edsair.doi.dedup.....862caff62b44fb69f2aad180dc1b4b00