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In vitro investigation of the genotoxicity of portimine, a cyclic imine toxin produced by the dinoflagellate Vulcanodinium rugosum, on human hepatic HepaRG cells
- Source :
- Toxicology in Vitro, Toxicology in Vitro, Elsevier, 2021, 73, pp.105125. ⟨10.1016/j.tiv.2021.105125⟩
- Publication Year :
- 2021
- Publisher :
- HAL CCSD, 2021.
-
Abstract
- International audience; Portimine, a recently identified cyclic imine produced by the dinoflagellate Vulcanodinium rugosum, has been described as a potent apoptotic agent in contrast to most of the cyclic imines that are well-known to be neurological toxins. As apoptosis can be a consequence of a high level of DNA lesions, we investigated the responses of portimine on several endpoints aimed at detecting DNA damage in the hepatic cell line HepaRG. Portimine induced phosphorylation of H2AX, which could possibly be consistent with the previously published induction of apoptosis with this toxin. In addition, detection of apoptosis through the activation of caspase-3, the induction of strand breaks detected by the comet assay as well as chromosome and genome mutations using the micronucleus assay were addressed. Surprisingly, portimine treatment resulted in increases in only γH2AX in differentiated HepaRG cells whereas no effects on the other endpoints were detected. These increases in γH2AX in the absence of genotoxic effects in the other tests could indicate that portimine could possibly induce a DNA replication stress and/or that the compound can be detoxified by the HepaRG cells.
- Subjects :
- 0301 basic medicine
Apoptosis
medicine.disease_cause
dinoflagellate
Histones
chemistry.chemical_compound
0302 clinical medicine
toxicité
Micronucleus Tests
Chemistry
dinoflagellé
General Medicine
3. Good health
in-vitro test
Liver
030220 oncology & carcinogenesis
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Micronucleus test
Dinoflagellida
Imines
essai comet
toxicology
DNA damage
Cell Survival
portimine
amine cyclique
cellule humlaine HepaRG
Cell Line
03 medical and health sciences
test in-vitro
comet assay
medicine
Humans
micronucleus
Spiro Compounds
human hepatic HepaRG cell
genotoxicity
toxicity
toxicologie
Molecular biology
In vitro
Comet assay
MESH: Imines
030104 developmental biology
génotoxicité
Marine Toxins
cyclic amine
Vulcanodinium rugosum
Micronucleus
DNA
Genotoxicity
DNA Damage
Subjects
Details
- Language :
- English
- ISSN :
- 08872333
- Database :
- OpenAIRE
- Journal :
- Toxicology in Vitro, Toxicology in Vitro, Elsevier, 2021, 73, pp.105125. ⟨10.1016/j.tiv.2021.105125⟩
- Accession number :
- edsair.doi.dedup.....8627e7de82b1ca15b36a40afe9209a5d