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Controlled adhesion and growth of long term glial and neuronal cultures on Parylene-C
- Source :
- PLoS ONE, Vol 6, Iss 9, p e25411 (2011), PLoS ONE, Delivopoulos, E & Murray, A F 2011, ' Controlled Adhesion and Growth of Long Term Glial and Neuronal Cultures on Parylene-C ', PLoS ONE, vol. 6, no. 9, e25411 . https://doi.org/10.1371/journal.pone.0025411
- Publication Year :
- 2011
- Publisher :
- Public Library of Science (PLoS), 2011.
-
Abstract
- This paper explores the long term development of networks of glia and neurons on patterns of Parylene-C on a SiO(2) substrate. We harvested glia and neurons from the Sprague-Dawley (P1-P7) rat hippocampus and utilized an established cell patterning technique in order to investigate cellular migration, over the course of 3 weeks. This work demonstrates that uncontrolled glial mitosis gradually disrupts cellular patterns that are established early during culture. This effect is not attributed to a loss of protein from the Parylene-C surface, as nitrogen levels on the substrate remain stable over 3 weeks. The inclusion of the anti-mitotic cytarabine (Ara-C) in the culture medium moderates glial division and thus, adequately preserves initial glial and neuronal conformity to underlying patterns. Neuronal apoptosis, often associated with the use of Ara-C, is mitigated by the addition of brain derived neurotrophic factor (BDNF). We believe that with the right combination of glial inhibitors and neuronal promoters, the Parylene-C based cell patterning method can generate structured, active neural networks that can be sustained and investigated over extended periods of time. To our knowledge this is the first report on the concurrent application of Ara-C and BDNF on patterned cell cultures.
- Subjects :
- Polymers
Cellular differentiation
lcsh:Medicine
Rats, Sprague-Dawley
0302 clinical medicine
Engineering
Molecular Cell Biology
lcsh:Science
Cells, Cultured
Medicine(all)
Neurons
0303 health sciences
Multidisciplinary
Agricultural and Biological Sciences(all)
Cytarabine
Cell migration
Cell Differentiation
Cell biology
medicine.anatomical_structure
Neuroglia
Cellular Types
Research Article
Biotechnology
Materials Science
Biomedical Engineering
Bioengineering
Biology
Xylenes
Medical Devices
Biomaterials
03 medical and health sciences
medicine
Cell Adhesion
Animals
Cell adhesion
Mitosis
030304 developmental biology
Cell Proliferation
Brain-derived neurotrophic factor
Tissue Engineering
Cell growth
Biochemistry, Genetics and Molecular Biology(all)
Brain-Derived Neurotrophic Factor
lcsh:R
Rats
nervous system
Cell culture
Immunology
Bionanotechnology
lcsh:Q
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 6
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....861842c2613b157d85a8f69c98f530f2