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Co-expression of protein complexes in prokaryotic and eukaryotic hosts: experimental procedures, database tracking and case studies
- Source :
- Acta Crystallographica Section D: Biological Crystallography, Acta Crystallographica Section D: Biological Crystallography, International Union of Crystallography, 2006, 62 (Pt 10), pp.1232-42. ⟨10.1107/S0907444906031003⟩, Acta Crystallographica Section D: Biological Crystallography, 62(10), 1232-1242. John Wiley & Sons Inc.
- Publication Year :
- 2006
- Publisher :
- HAL CCSD, 2006.
-
Abstract
- Structure determination and functional characterization of macromolecular complexes requires the purification of the different subunits in large quantities and their assembly into a functional entity. Although isolation and structure determination of endogenous complexes has been reported, much progress has to be made to make this technology easily accessible. Co-expression of subunits within hosts such as Escherichia coli and insect cells has become more and more amenable, even at the level of high-throughput projects. As part of SPINE (Structural Proteomics In Europe), several laboratories have investigated the use co-expression techniques for their projects, trying to extend from the common binary expression to the more complicated multi-expression systems. A new system for multi-expression in E. coli and a database system dedicated to handle co-expression data are described. Results are also reported from various case studies investigating different methods for performing co-expression in E. coli and insect cells.
- Subjects :
- Insecta
DNA Repair
Information Management
Receptors, Cytoplasmic and Nuclear
computer.software_genre
medicine.disease_cause
MESH: Eukaryotic Cells
MESH: Recombinant Proteins
MESH: Insects
Structural Biology
MESH: Genetic Vectors
Databases, Genetic
MESH: Animals
MESH: Databases, Genetic
SPINE (molecular biology)
MESH: DNA Repair
0303 health sciences
Database
MESH: Escherichia coli
030302 biochemistry & molecular biology
MESH: Transcription Factor TFIID
General Medicine
Cyclin-Dependent Kinases
Recombinant Proteins
Eukaryotic Cells
MESH: Cyclin-Dependent Kinases
Macromolecular Complexes
MESH: Information Management
Algorithms
Ubiquitin-Protein Ligases
Genetic Vectors
MESH: Computer Security
MESH: Algorithms
Biology
MESH: Receptors, Cytoplasmic and Nuclear
03 medical and health sciences
MESH: Computer Simulation
MESH: RNA
Structural proteomics
medicine
Escherichia coli
Animals
Computer Simulation
Computer Security
030304 developmental biology
MESH: Prokaryotic Cells
Insect cell
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
Expression (computer science)
MESH: Ubiquitin-Protein Ligases
Prokaryotic Cells
RNA
Transcription Factor TFIID
computer
Cyclin-Dependent Kinase-Activating Kinase
Subjects
Details
- Language :
- English
- ISSN :
- 09074449
- Database :
- OpenAIRE
- Journal :
- Acta Crystallographica Section D: Biological Crystallography, Acta Crystallographica Section D: Biological Crystallography, International Union of Crystallography, 2006, 62 (Pt 10), pp.1232-42. ⟨10.1107/S0907444906031003⟩, Acta Crystallographica Section D: Biological Crystallography, 62(10), 1232-1242. John Wiley & Sons Inc.
- Accession number :
- edsair.doi.dedup.....860d9aae1aed0b057563fdb6ed820dc2