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Taxonomic and functional prokaryote diversity in mildly arsenic-contaminated sediments

Authors :
David Halter
Florence Goulhen-Chollet
Audrey Cordi
Fabienne Séby
Christophe Pagnout
Christine Schaeffer
Philippe N. Bertin
Sebastien Gallien
Simonetta Gribaldo
Didier Montaut
Florence Arsène-Ploetze
Pascale Bauda
Alain Van Dorsselaer
Christine Carapito
Audrey Heinrich-Salmeron
Génétique moléculaire, génomique, microbiologie (GMGM)
Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)
Laboratoire des Interactions Ecotoxicologie, Biodiversité, Ecosystèmes (LIEBE)
Université Paul Verlaine - Metz (UPVM)-Centre National de la Recherche Scientifique (CNRS)
Biologie Moléculaire du Gène chez les Extrêmophiles (BMGE)
Institut Pasteur [Paris] (IP)
Laboratoire de Spectrométrie de Masse BioOrganique [Strasbourg] (LSMBO)
Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC)
Institut Pluridisciplinaire Hubert Curien (IPHC)
Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Institut Pluridisciplinaire Hubert Curien (IPHC)
Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)
Ultra Traces Analyses Aquitaine (UT2A)
Ultra Traces Analyses Aquitaine
Audrey Cordi was supported by grants from the French Ministry of Research and the 'Zone Atelier Moselle' (ZAM) project. Audrey Heinrich-Salmeron, David Halter and Sebastien Gallien were supported by grants from the French Ministry of Research and the Université de Strasbourg. Florence Goulhen-Chollet was supported by the Agence Nationale de la Recherche (ANR), RARE project (Reactivity of an Arsenic-Rich Ecosystem). This study was financed by the EC2CO program (Institut National des Sciences de l’Univers, CNRS) and BRG.
This work was performed in the framework of the Groupement de Recherche 'Métabolisme de l’Arsenic chez les micro-organismes: de la résistance à la détoxication' (GDR2909-CNRS).
ANR-07-BLAN-0118,RARE,Reactivity of an arsenic-rich ecosystem: an integrated genomics approach(2007)
Institut Pasteur [Paris]
Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut Pluridisciplinaire Hubert Curien (IPHC)
Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)
Source :
Research in Microbiology, Research in Microbiology, 2011, 162 (9), pp.877-887. ⟨10.1016/j.resmic.2011.06.001⟩, Research in Microbiology, Elsevier, 2011, 162 (9), pp.877-887. ⟨10.1016/j.resmic.2011.06.001⟩
Publication Year :
2011
Publisher :
HAL CCSD, 2011.

Abstract

International audience; Arsenic-resistant prokaryote diversity is far from being exhaustively explored. In this study, the arsenic-adapted prokaryotic community present in a moderately arsenic-contaminated site near Sainte-Marie-aux-Mines (France) was characterized, using metaproteomic and 16S rRNA-encoding gene amplification. High prokaryotic diversity was observed, with a majority of Proteobacteria, Acidobacteria and Bacter-oidetes, and a large archaeal community comprising Euryarchaeaota and Thaumarchaeota. Metaproteomic analysis revealed that Proteobacteria, Planctomycetes and Cyanobacteria are among the active bacteria in this ecosystem. Taken together, these results highlight the unsuspected high diversity of the arsenic-adapted prokaryotic community, with some phyla never having been described in highly arsenic-exposed sites.

Subjects

Subjects :
Proteomics
Geologic Sediments
Thaumarchaeota
MESH: Archaea/metabolism
MESH: Environmental Pollutants/metabolism
[SDV]Life Sciences [q-bio]
MESH: Bacteria/genetics
MESH: Archaea/classification
Polymerase Chain Reaction
MESH: DNA, Archaeal/analysis
RNA, Ribosomal, 16S
MESH: Archaea/isolation & purification
MESH: Ecosystem
MESH: Geologic Sediments/microbiology
MESH: Phylogeny
Phylogeny
0303 health sciences
biology
Ecology
Planctomycetes
MESH: Microbial Consortia/physiology
General Medicine
Biodiversity
Adaptation, Physiological
DNA, Archaeal
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
MESH: Arsenic/metabolism
Environmental Pollutants
France
Proteobacteria
MESH: Archaea/genetics
MESH: Bacteria/metabolism
DNA, Bacterial
MESH: Rivers/microbiology
MESH: RNA, Ribosomal, 16S/analysis
Gene Transfer, Horizontal
Microbial Consortia
Environmental genomics
Microbiology
Mining
MESH: Biodiversity
Arsenic
03 medical and health sciences
Rivers
Metaproteomics
MESH: DNA, Bacterial/analysis
Molecular Biology
Ecosystem
030304 developmental biology
MESH: Proteomics
Bacteria
030306 microbiology
Phylum
Bacteroidetes
Prokaryote
MESH: Polymerase Chain Reaction
biology.organism_classification
Archaea
MESH: Adaptation, Physiological
MESH: RNA, Ribosomal, 16S/genetics
MESH: Gene Transfer, Horizontal
MESH: DNA, Bacterial/genetics
MESH: France
Metagenomics
MESH: DNA, Archaeal/genetics
MESH: Bacteria/classification
human activities
Acidobacteria
MESH: Bacteria/isolation & purification

Details

Language :
English
ISSN :
09232508
Database :
OpenAIRE
Journal :
Research in Microbiology, Research in Microbiology, 2011, 162 (9), pp.877-887. ⟨10.1016/j.resmic.2011.06.001⟩, Research in Microbiology, Elsevier, 2011, 162 (9), pp.877-887. ⟨10.1016/j.resmic.2011.06.001⟩
Accession number :
edsair.doi.dedup.....86091aef806c17c79972c59f0426a3b2
Full Text :
https://doi.org/10.1016/j.resmic.2011.06.001⟩
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