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Low serum Kallistatin level was associated with poor neurological outcome of out-of-hospital cardiac arrest survivors: Proteomics study
- Source :
- Resuscitation. 128
- Publication Year :
- 2018
-
Abstract
- Aim of the study To identify proteins of which depletion are associated with the poor 6-month neurological outcome of out-of-hospital cardiac arrest survivors. Methods Seven healthy volunteers and 34 out-of-hospital cardiac arrest survivors admitted to the intensive care unit (ICU) and underwent targeted-temperature management were enrolled. According to the 6-month cerebral performance category (CPC) scale, patients were divided into the good (CPC 1–2) and poor (CPC 3–5) outcome groups. Blood samples were obtained at 0, 24, and 72 h after admission to the ICU. Results With proteomic approaches, we found 23 proteins that showed group-differences between the sera pooled from 7 study groups: healthy volunteers, the good outcome groups (0, 24, and 72 h), and the poor outcome groups (0, 24, and 72 h). We selected 7 candidate proteins of which intensities were different between the good and poor outcome groups (>2-fold change) and excluded 5 proteins related to haemolysis or remaining high abundant proteins. To confirm the 2 identified proteins: retinal dehydrogenase 1 and Kallistatin, we performed enzyme-linked immunosorbent assay with individual serum. Finally, old age (odds ratio = 1.055; 95% confidence interval, 1.002–1.112; p = 0.043) and low serum kallistatin level at 0 h (odds ratio = 0.784; 95% confidence interval, 0.618–0.995; p = 0.046) were independently associated with the poor 6-month neurological outcome. Conclusion The depletion of serum kallistatin at admission to the ICU was associated with the poor neurological outcome of out-of-hospital cardiac arrest survivors.
- Subjects :
- Adult
Proteomics
medicine.medical_specialty
Enzyme-Linked Immunosorbent Assay
030204 cardiovascular system & hematology
Emergency Nursing
Out of hospital cardiac arrest
law.invention
03 medical and health sciences
0302 clinical medicine
law
Hypothermia, Induced
Internal medicine
Healthy volunteers
RETINAL DEHYDROGENASE 1
Medicine
Humans
Serpins
Analysis of Variance
business.industry
Age Factors
Retinal Dehydrogenase
030208 emergency & critical care medicine
Recovery of Function
Hypothermia
Haemolysis
Intensive care unit
Confidence interval
Cardiopulmonary Resuscitation
Kallistatin
Case-Control Studies
Emergency Medicine
medicine.symptom
Cardiology and Cardiovascular Medicine
business
Biomarkers
Out-of-Hospital Cardiac Arrest
Subjects
Details
- ISSN :
- 18731570
- Volume :
- 128
- Database :
- OpenAIRE
- Journal :
- Resuscitation
- Accession number :
- edsair.doi.dedup.....86069af436d9f1cde88c01ac14af85d3