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Graphene Oxide Nanoparticles Modified Paper Electrode as a Biosensing Platform for Detection of the htrA Gene of O. tsutsugamushi
- Source :
- Sensors (Basel, Switzerland), Sensors, Vol 21, Iss 4366, p 4366 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- The unique structural and electrochemical properties of graphene oxide (GO) make it an ideal material for the fabrication of biosensing devices. Therefore, in the present study, graphene oxide nanoparticles modified paper electrodes were used as a low-cost matrix for the development of an amperometric DNA sensor. The graphene oxide was synthesized using the modified hummers method and drop cast on a screen-printed paper electrode (SPPE) to enhance its electrochemical properties. Further, the GO/SPPE electrode was modified with a 5′NH2 labeled ssDNA probe specific to the htrA gene of Orientia tsutsugamushi using carbodiimide cross-linking chemistry. The synthesized GO was characterized using UV-Vis, FTIR, and XRD. The layer-by-layer modification of the paper electrode was monitored via FE-SEM, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS). The sensor response after hybridization with single-stranded genomic DNA (ssGDNA) of O. tsutsugamushi was recorded using differential pulse voltammetry (DPV). Methylene blue (1 mM in PBS buffer, pH 7.2) was used as a hybridization indicator and [Fe(CN)6]−3/−4 (2.5 mM in PBS buffer, pH 7.2) as a redox probe during electrochemical measurements. The developed DNA sensor shows excellent sensitivity (1228.4 µA/cm2/ng) and LOD (20 pg/µL) for detection of O. tsutsugamushi GDNA using differential pulse voltammetry (DPV).
- Subjects :
- Materials science
Oxide
DNA sensor
TP1-1185
02 engineering and technology
Biosensing Techniques
htrA gene
01 natural sciences
Biochemistry
Analytical Chemistry
law.invention
chemistry.chemical_compound
law
Electrical and Electronic Engineering
Instrumentation
Electrodes
scrub typhus
Graphene
Chemical technology
Communication
010401 analytical chemistry
electrochemical
021001 nanoscience & nanotechnology
Atomic and Molecular Physics, and Optics
Amperometry
0104 chemical sciences
Dielectric spectroscopy
chemistry
Electrode
screen printed paper electrode
Nanoparticles
Graphite
Differential pulse voltammetry
Cyclic voltammetry
0210 nano-technology
Biosensor
Nuclear chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 14248220
- Volume :
- 21
- Issue :
- 13
- Database :
- OpenAIRE
- Journal :
- Sensors (Basel, Switzerland)
- Accession number :
- edsair.doi.dedup.....85f5e774ccf366ebae9cdfa03a40b9d0