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The Arabidopsis lectin receptor kinase LecRK-I.9 enhances resistance to Phytophthora infestans in Solanaceous plants
- Source :
- Plant Biotechnology Journal 12 (2014) 1, Plant Biotechnology Journal, 12(1), 10-16
- Publication Year :
- 2014
-
Abstract
- Late blight caused by the plant pathogenic oomycete Phytophthora infestans is known as one of the most destructive potato diseases. Plant breeders tend to employ NB-LRR-based resistance for introducing genetically controlled late blight resistance in their breeding lines. However, P. infestans is able to rapidly escape this type of resistance, and hence, NB-LRR-based resistance in potato cultivars is often not durable. Previously, we identified a novel type of Phytophthora resistance in Arabidopsis. This resistance is mediated by the cell surface receptor LecRK-I.9, which belongs to the family of L-type lectin receptor kinases. In this study, we report that expression of the Arabidopsis LecRK-I.9 gene in potato and Nicotiana benthamiana results in significantly enhanced late blight resistance. Transcriptional profiling showed strong reduction in salicylic acid (SA)-mediated defence gene expression in LecRK-I.9 transgenic potato lines (TPLs). In contrast, transcripts of two protease inhibitor genes accumulated to extreme high levels, suggesting that LecRK-I.9-mediated late blight resistance is relying on a defence response that includes activation of protease inhibitors. These results demonstrate that the functionality of LecRK-I.9 in Phytophthora resistance is maintained after interfamily transfer to potato and N. benthamiana and suggest that this novel type of LecRK-based resistance can be exploited in breeding strategies to improve durable late blight resistance in Solanaceous crops.
- Subjects :
- Phytophthora infestans
Arabidopsis
Nicotiana benthamiana
Plant Science
Plant disease resistance
potato cultivars
Gene Expression Regulation, Plant
PRI Biodiversiteit en Veredeling
Botany
expression
Blight
Laboratorium voor Plantenfysiologie
gene
Gene
Plant Diseases
Plant Proteins
Solanum tuberosum
Genetics
Oomycete
lacking
biology
EPS-2
fungi
food and beverages
plasma-membrane
Plants, Genetically Modified
biology.organism_classification
infection
proteins
Laboratorium voor Phytopathologie
interfamily transfer
PRI Biodiversity and Breeding
Laboratory of Phytopathology
activation
Phytophthora
Agronomy and Crop Science
Laboratory of Plant Physiology
Biotechnology
tomato ve1
Subjects
Details
- Language :
- English
- ISSN :
- 14677644
- Database :
- OpenAIRE
- Journal :
- Plant Biotechnology Journal 12 (2014) 1, Plant Biotechnology Journal, 12(1), 10-16
- Accession number :
- edsair.doi.dedup.....85ea921b0707ee8995ec72bfe1ec88f3