Back to Search
Start Over
Identification of the most abundant proteins in equine amniotic fluid by a proteomic approach
- Publication Year :
- 2016
-
Abstract
- Characterisation of the physiologic equine amniotic fluid (AF) proteome is a prerequisite to study its changes during diseases and discover new biomarkers. The aim of this study was to identify by a proteomic approach the most abundant proteins of equine AF. AF samples were collected at parturition from 24 healthy mares that delivered healthy foals. All samples were subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) on 4–12% gels. A pool of the 24 samples, after SDS-PAGE, was cut in 25 slices, trypsin-digested and analysed by mass spectrometry (MS) for protein identification. Mean AF protein concentration was 1.96±1.12g/L. Thirty-four proteins were successfully identified by MS and subsequently categorised according to Gene Ontology (GO). Twelve proteins (e.g. fibronectin, lumican, thrombospondin and fibulin) belonged to or interacted with the extracellular matrix (ECM) playing an important role in the development of foetal tissues. Most of the remaining proteins were classified as transport (e.g. albumin, major allergen Equ c1 and alpha-fetoprotein) delivering nutrients, ions and lipids essential for foetal growth and development. Among these proteins, major allergen Equ c1 is widely studied in human medicine because it induces Ig-E mediated type I allergic reaction. The absence of immunoglobulins in equine AF was also confirmed.
- Subjects :
- Proteomics
0301 basic medicine
Amniotic fluid
Proteome
Lumican
Biology
Horse
03 medical and health sciences
0302 clinical medicine
Endocrinology
Food Animals
Electrophoresi
Pregnancy
Animals
Horses
Polyacrylamide gel electrophoresis
Electrophoresis
Animal Science and Zoology
Thrombospondin
030219 obstetrics & reproductive medicine
Albumin
Proteins
General Medicine
Amniotic Fluid
Molecular biology
Fibulin
Fibronectin
030104 developmental biology
biology.protein
Female
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....85e0c9fe81e56c3d70e0459f541f59bb