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Optogenetic precision toolkit to reveal form, function and connectivity of single neurons
- Source :
- Methods. 150:42-48
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- All-optical methods enable the control and monitoring of neuronal activity with minimal perturbation of the system. Although imaging and optogenetic manipulations can be performed at cellular resolution, the morphology of single cells in a dense neuronal population has often remained unresolvable. Here we describe in detail two recently established optogenetic protocols for systematic description of function and morphology of single neurons in zebrafish. First, the Optobow toolbox allows unbiased mapping of excitatory functional connectivity. Second, the FuGIMA technique enables selective labeling and anatomical tracing of neurons that are responsive to a given sensory stimulus or correlated with a specific behavior. Both strategies can be genetically targeted to a neuronal population of choice using the Gal4/UAS system. As these in vivo approaches are non-invasive, we envision useful applications for the study of neuronal structure, function and connectivity during development and behavior.
- Subjects :
- 0301 basic medicine
Computer science
Green Fluorescent Proteins
Biosensing Techniques
Optogenetics
Tracing
General Biochemistry, Genetics and Molecular Biology
Animals, Genetically Modified
03 medical and health sciences
Two-photon excitation microscopy
Biological neural network
Animals
Premovement neuronal activity
Molecular Biology
Zebrafish
Neurons
biology
Brain
Zebrafish Proteins
biology.organism_classification
DNA-Binding Proteins
Enhancer Elements, Genetic
030104 developmental biology
Cellular resolution
Excitatory postsynaptic potential
Calcium
Nerve Net
Neuroscience
Photic Stimulation
Transcription Factors
Subjects
Details
- ISSN :
- 10462023
- Volume :
- 150
- Database :
- OpenAIRE
- Journal :
- Methods
- Accession number :
- edsair.doi.dedup.....85c44725de7986a94cff91a16932f73b