Back to Search
Start Over
Application of a robust microplate assay to determine induced β-1,3-glucanase and chitinase activity in the cotton plant
- Source :
- BioTechniques. 70(4)
- Publication Year :
- 2021
-
Abstract
- Resistance is induced in cotton plants as the result of either viral infection or exogenous application of elicitors. Induced resistance can be evaluated by determining the production of β-1,3-glucanase and chitinase in plants as a biochemical parameter. The assays being used for the determination of chitinase and β-1,3-glucanase activity are laborious and not cost-effective, as the reducing sugars produced by the substrates colloidal chitin and laminarin are very expensive. The concentration of both substrates was standardized and reduced to 0.25% from 4% in a modified microplate assay, which appeared to be more effective. The amount of β-1,3-glucanase and chitinase produced was significant and determined by the new modified assay. The sensitivity of the microplate assay was significantly raised approximately one- to twofold.
- Subjects :
- 0301 basic medicine
Gossypium
biology
Chemistry
Chitinases
food and beverages
Chitin
Chitinase activity
Glucan 1,3-beta-Glucosidase
Glucanase
Viral infection
General Biochemistry, Genetics and Molecular Biology
Microbiology
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
030220 oncology & carcinogenesis
Chitinase
biology.protein
Biotechnology
Subjects
Details
- ISSN :
- 19409818
- Volume :
- 70
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- BioTechniques
- Accession number :
- edsair.doi.dedup.....85c11a1936abb1000ee16cd12cbc3730