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Differential expression of HIV envelope epitopes on the surface of HIV-Infected macrophages and CD4+ T cells
- Source :
- Antiviral Research. 191:105085
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- HIV-infected macrophages contribute to persistence of HIV reservoirs in people living with HIV receiving antiretroviral therapy. A potential strategy to eliminate reservoirs is the use of antibody-dependent cellular cytotoxicity (ADCC) against infected cells expressing the HIV envelope (Env) protein on their surface. Designing ADCC strategies requires knowledge of exposed Env epitopes on the cell surface and identifying antibodies capable of opsonising infected cells, yet little is known regarding the ability of HIV-infected macrophages to be targeted with such strategies. Using a panel of neutralising and poorly-neutralising anti-Env antibodies we compared Env epitopes expressed on infected monocyte-derived macrophages (MDM) and autologous T cells. Our results reveal potential differences in epitope expression on macrophage- and T cell-expressed Env. Notably, HIVBaL-infected macrophages were more susceptible to opsonisation by NIH45-46 (median = 40.4%) compared to infected T cells (13.6%; p = 0.002), which were more susceptible to opsonisation by 17b and 447.52D (88.6% and 45.6% respectively) compared to MDM (30% and 6.7%, p = 0.002 and 0.004 respectively). Furthermore, neutralising antibodies 10E8 and PGT145 were relatively ineffective at opsonising Env expressed on the surface of infected T cells or macrophages, indicating that the context in which Env is presented on infected cells may differ to that of cell-free virions.
- Subjects :
- CD4-Positive T-Lymphocytes
0301 basic medicine
030106 microbiology
Cell
Human immunodeficiency virus (HIV)
Context (language use)
HIV Antibodies
Biology
medicine.disease_cause
Epitope
Epitopes
03 medical and health sciences
Virology
medicine
Humans
Macrophage
Cells, Cultured
Pharmacology
Antibody-dependent cell-mediated cytotoxicity
Macrophages
Antibody-Dependent Cell Cytotoxicity
env Gene Products, Human Immunodeficiency Virus
virus diseases
030104 developmental biology
medicine.anatomical_structure
HIV-1
biology.protein
Antibody
Hiv envelope
Protein Binding
Subjects
Details
- ISSN :
- 01663542
- Volume :
- 191
- Database :
- OpenAIRE
- Journal :
- Antiviral Research
- Accession number :
- edsair.doi.dedup.....85b25760512450b36fba415717b88009