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Chronic Salmonella Infection Induced Intestinal Fibrosis
- Source :
- Journal of visualized experiments : JoVE. (151)
- Publication Year :
- 2019
-
Abstract
- Tissue fibrosis characterized by the pathological accumulation of extracellular matrix such as collagen is the outcome of persistent inflammation and dysregulated repair. In inflammatory bowel disease (IBD), fibrosis leads to recurrent stricture formations for which there is no effective therapy other than surgical resection. Due to its late onset, the processes that drive fibrosis is less studied and largely unknown. Therefore, fibrotic complications represent a major challenge in IBD. In this protocol, a robust in vivo model of intestinal fibrosis is described where streptomycin pre-treatment of C57Bl/6 mice followed by oral gavage with vaccine grade Salmonella Typhimurium ΔAroA mutant leads to persistent pathogen colonization and fibrosis of the cecum. Methodologies for preparing S. Typhimurium ΔAroA for inoculation, quantifying pathogen loads in the cecum and spleen, and evaluating collagen deposition in intestinal tissues are explained. This experimental disease model is useful for examining host factors that either enhance or exacerbate CD-like intestinal fibrosis.
- Subjects :
- Salmonella typhimurium
General Chemical Engineering
Spleen
Salmonella infection
Inflammatory bowel disease
General Biochemistry, Genetics and Molecular Biology
Extracellular matrix
Cecum
Fibrosis
medicine
Animals
Intestinal Mucosa
Pathogen
Inflammation
Crohn's disease
General Immunology and Microbiology
business.industry
General Neuroscience
medicine.disease
Intestines
Mice, Inbred C57BL
medicine.anatomical_structure
Immunology
Chronic Disease
Salmonella Infections
Cytokines
Collagen
business
Subjects
Details
- ISSN :
- 1940087X
- Issue :
- 151
- Database :
- OpenAIRE
- Journal :
- Journal of visualized experiments : JoVE
- Accession number :
- edsair.doi.dedup.....858d82617f3afb4f1bcd7302620ba3f4