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Vaccenic acid suppresses intestinal inflammation by increasing anandamide and related N-acylethanolamines in the JCR:LA-cp rat[S]
- Source :
- Journal of Lipid Research, Vol 57, Iss 4, Pp 638-649 (2016), Jacome-Sosa, M; Vacca, C; Mangat, R; Diane, A; Nelson, RC; Reaney, MJ; et al.(2016). Vaccenic acid suppresses intestinal inflammation by increasing anandamide and related N-acylethanolamines in the JCR:LA-cp rat. JOURNAL OF LIPID RESEARCH, 57(4), 638-649. doi: 10.1194/jlr.M066308. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/10k3v3s8, Journal of Lipid Research
- Publication Year :
- 2016
- Publisher :
- Elsevier, 2016.
-
Abstract
- Vaccenic acid (VA), the predominant ruminant-derived trans fat in the food chain, ameliorates hyperlipidemia, yet mechanisms remain elusive. We investigated whether VA could influence tissue endocannabinoids (ECs) by altering the availability of their biosynthetic precursor, arachidonic acid (AA), in membrane phospholipids (PLs). JCR:LA-cp rats were assigned to a control diet with or without VA (1% w/w), cis-9, trans-11 conjugated linoleic acid (CLA) (1% w/w) or VA+CLA (1% + 0.5% w/w) for 8 weeks. VA reduced the EC, 2-arachidonoylglycerol (2-AG), in the liver and visceral adipose tissue (VAT) relative to control diet (P < 0.001), but did not change AA in tissue PLs. There was no additive effect of combining VA+CLA on 2-AG relative to VA alone (P > 0.05). Interestingly, VA increased jejunal concentrations of anandamide and those of the noncannabinoid signaling molecules, oleoylethanolamide and palmitoylethanolamide, relative to control diet (P < 0.05). This was consistent with a lower jejunal protein abundance (but not activity) of their degrading enzyme, fatty acid amide hydrolase, as well as the mRNA expression of TNFα and interleukin 1β (P < 0.05). The ability of VA to reduce 2-AG in the liver and VAT provides a potential mechanistic explanation to alleviate ectopic lipid accumulation. The opposing regulation of ECs and other noncannabinoid lipid signaling molecules by VA suggests an activation of benefit via the EC system in the intestine.
- Subjects :
- Male
0301 basic medicine
Conjugated linoleic acid
Anti-Inflammatory Agents
Vaccenic acid
Oleic Acids
Biochemistry
Oleoylethanolamide
chemistry.chemical_compound
Endocrinology
Fatty acid amide hydrolase
anandamide
Intestinal Mucosa
Research Articles
Metabolic Syndrome
Anandamide
Intestines
Liver
Ethanolamines
Cytokines
Arachidonic acid
medicine.medical_specialty
Polyunsaturated Alkamides
Arachidonic Acids
QD415-436
Intra-Abdominal Fat
Biology
Gene Expression Regulation, Enzymologic
Amidohydrolases
Membrane Lipids
03 medical and health sciences
Internal medicine
medicine
Animals
Humans
RNA, Messenger
endocannabinoids
Inflammation
Palmitoylethanolamide
intestinal inflammatory diseases
Cell Biology
Lipid signaling
Rats
N-acylethanolamines
Disease Models, Animal
030104 developmental biology
chemistry
Dietary Supplements
Caco-2 Cells
vaccenic acid
Subjects
Details
- Language :
- English
- ISSN :
- 00222275
- Volume :
- 57
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of Lipid Research
- Accession number :
- edsair.doi.dedup.....858a7664375c1ca0b8e7ae8e4256647a