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Detection of a pheromone-binding protein in the aquatic fungus Achlya ambisexualis
- Source :
- Experimental Cell Research. 153:544-549
- Publication Year :
- 1984
- Publisher :
- Elsevier BV, 1984.
-
Abstract
- Sexual reproduction among the eukaryotic fungi of the genus, Achlya, is controlled by two steroid pheromones. Antheridiol is the pheromone constitutively produced by female cells that induces male sexual differentiation and development. A biologically active tritium-labeled derivative of antheridiol, [1,2-3H]7-deoxy-7-dihydro-antheridiol ( [3H]7dA), has been synthesized. Radioligand-binding studies have revealed the presence of a specific binding protein in the cytosol of male cells that may represent the endogenous receptor for antheridiol. Binding to this macromolecule was characterized by an apparent equilibrium dissociation constant and maximum binding capacity of approx. 7 X 10(-10) M and 1 100-2 000 fmoles/mg protein, respectively. Sedimentation analysis in sucrose gradients revealed that the binding protein distributes in the 8S region under low ionic strength and sodium molybdate-stabilized conditions. Under conditions of high ionic strength, in the presence or absence of 10 mM sodium molybdate, the binding site distributes in the 3.6S region of the gradient. Analysis of radioligand binding in the presence of other steroids and steroid hormones revealed that the binding is specific for antheridiol and its analog.
- Subjects :
- Cytoplasm
Binding protein
medicine.medical_treatment
Fungi
Phytosterols
Cell Biology
Achlya
Plasma protein binding
Biology
biology.organism_classification
Chemoreceptor Cells
Steroid
Dissociation constant
Cytosol
Biochemistry
Centrifugation, Density Gradient
medicine
Binding site
Pheromone binding protein
Protein Binding
Subjects
Details
- ISSN :
- 00144827
- Volume :
- 153
- Database :
- OpenAIRE
- Journal :
- Experimental Cell Research
- Accession number :
- edsair.doi.dedup.....85891ecc7ffc785d9593a934d60bdc5a