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Quantum Capacitance-Limited MoS(2) Biosensors Enable Remote Label-Free Enzyme Measurements
- Source :
- Nanoscale
- Publication Year :
- 2019
-
Abstract
- We have demonstrated atomically thin, quantum capacitance-limited, field-effect transistors (FETs) that enable the detection of pH changes with 75-fold higher sensitivity (≈4.4 V/pH) over the Nernst value of 59 mV/pH at room temperature when used as a biosensor. The transistors, which are fabricated from monolayer films of MoS(2), use a room temperature ionic liquid (RTIL) in place of a conventional oxide gate dielectric and exhibit very low intrinsic noise resulting in a pH resolution of 92×10(−6) at 10 Hz. This high device performance, which is a function of the structure of our device, is achieved by remotely connecting the gate to a pH sensing element allowing the FETs to be reused. Because pH measurements are fundamentally important in biotechnology, the increased resolution demonstrated here will benefit numerous applications ranging from pharmaceutical manufacturing to clinical diagnostics. As an example, we experimentally quantified the function of the kinase Cdk5, an enzyme implicated in Alzheimer’s disease, at concentrations that are 5-fold lower than physiological values, and with sufficient time-resolution to allow the estimation of both steady-state and kinetic parameters in a single experiment. The high sensitivity, increased resolution, and fast turnaround time of the measurements will allow the development of early diagnostic tools and novel therapeutics to detect and treat neurological conditions years before currently possible.
- Subjects :
- Materials science
Transistors, Electronic
Gate dielectric
FOS: Physical sciences
Ionic Liquids
02 engineering and technology
Applied Physics (physics.app-ph)
Biosensing Techniques
Signal-To-Noise Ratio
010402 general chemistry
Electric Capacitance
01 natural sciences
Noise (electronics)
Quantitative Biology - Quantitative Methods
Article
law.invention
Quantum capacitance
symbols.namesake
law
Alzheimer Disease
Limit of Detection
Humans
General Materials Science
Nernst equation
Disulfides
Quantitative Methods (q-bio.QM)
Detection limit
Molybdenum
business.industry
Transistor
Temperature
Cyclin-Dependent Kinase 5
Physics - Applied Physics
Hydrogen-Ion Concentration
021001 nanoscience & nanotechnology
0104 chemical sciences
Kinetics
FOS: Biological sciences
symbols
Optoelectronics
0210 nano-technology
business
Sensitivity (electronics)
Biosensor
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Nanoscale
- Accession number :
- edsair.doi.dedup.....8585e67c2b3c1c31eb975ad24ec86960