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A role for worm cutl-24 in background- and parent-of-origin-dependent ER stress resistance
- Source :
- BMC genomics, vol 23, iss 1
- Publication Year :
- 2022
- Publisher :
- Springer Science and Business Media LLC, 2022.
-
Abstract
- Background Organisms in the wild can acquire disease- and stress-resistance traits that outstrip the programs endogenous to humans. Finding the molecular basis of such natural resistance characters is a key goal of evolutionary genetics. Standard statistical-genetic methods toward this end can perform poorly in organismal systems that lack high rates of meiotic recombination, like Caenorhabditis worms. Results Here we discovered unique ER stress resistance in a wild Kenyan C. elegans isolate, which in inter-strain crosses was passed by hermaphrodite mothers to hybrid offspring. We developed an unbiased version of the reciprocal hemizygosity test, RH-seq, to explore the genetics of this parent-of-origin-dependent phenotype. Among top-scoring gene candidates from a partial-coverage RH-seq screen, we focused on the neuronally-expressed, cuticlin-like gene cutl-24 for validation. In gene-disruption and controlled crossing experiments, we found that cutl-24 was required in Kenyan hermaphrodite mothers for ER stress tolerance in their inter-strain hybrid offspring; cutl-24 was also a contributor to the trait in purebred backgrounds. Conclusions These data establish the Kenyan strain allele of cutl-24 as a determinant of a natural stress-resistant state, and they set a precedent for the dissection of natural trait diversity in invertebrate animals without the need for a panel of meiotic recombinants.
- Subjects :
- Bioinformatics
Biological Sciences
Kenya
Medical and Health Sciences
Phenotype
Caenorhabdis elegans
Information and Computing Sciences
Caenorhabditis
Genetics
Animals
Humans
Antimicrobial Resistance
Stress resistance
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Statistical genetics
Biotechnology
Subjects
Details
- ISSN :
- 14712164
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- BMC Genomics
- Accession number :
- edsair.doi.dedup.....85793b2e66163bc031ef3c3ef4bffd9f