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Chlamydia muridarum infection differentially alters smooth muscle function in mouse uterine horn and cervix
- Source :
- American Journal of Physiology-Endocrinology and Metabolism. 318:E981-E994
- Publication Year :
- 2020
- Publisher :
- American Physiological Society, 2020.
-
Abstract
- Chlamydia trachomatis infection is a primary cause of reproductive tract diseases including infertility. Previous studies showed that this infection alters physiological activities in mouse oviducts. Whether this occurs in the uterus and cervix has never been investigated. This study characterized the physiological activities of the uterine horn and the cervix in a Chlamydia muridarum ( Cmu)-infected mouse model at three infection time points of 7, 14, and 21 days postinfection (dpi). Cmu infection significantly decreased contractile force of spontaneous contraction in the cervix (7 and 14 dpi; P < 0.001 and P < 0.05, respectively), but this effect was not observed in the uterine horn. The responses of the uterine horn and cervix to oxytocin were significantly altered by Cmu infection at 7 dpi ( P < 0.0001), but such responses were attenuated at 14 and 21 dpi. Cmu infection increased contractile force to prostaglandin (PGF2α) by 53–83% in the uterine horn. This corresponded with the increased messenger ribonucleic acid (mRNA) expression of Ptgfr that encodes for its receptor. However, Cmu infection did not affect contractions of the uterine horn and cervix to PGE2 and histamine. The mRNA expression of Otr and Ptger4 was inversely correlated with the mRNA expression of Il1b, Il6 in the uterine horn of Cmu-inoculated mice ( P < 0.01 to P < 0.001), suggesting that the changes in the Otr and Ptger4 mRNA expression might be linked to the changes in inflammatory cytokines. Lastly, this study also showed a novel physiological finding of the differential response to PGE2 in mouse uterine horn and cervix.
- Subjects :
- 0301 basic medicine
Physiology
Endocrinology, Diabetes and Metabolism
Receptors, Prostaglandin
Interleukin-1beta
Uterus
Cervix Uteri
Oviducts
Dinoprost
Reproductive Tract Infections
Uterine contraction
Mice
Uterine Contraction
chemistry.chemical_compound
0302 clinical medicine
Oxytocics
Receptor
030219 obstetrics & reproductive medicine
biology
Uterine horns
medicine.anatomical_structure
Receptors, Oxytocin
06 Biological Sciences, 11 Medical and Health Sciences
Myometrium
Cytokines
Female
medicine.symptom
Histamine
Muscle Contraction
medicine.drug
Chlamydia muridarum
medicine.medical_specialty
Prostaglandin
Dinoprostone
Histamine Agonists
Endocrinology & Metabolism
03 medical and health sciences
Physiology (medical)
Internal medicine
medicine
Animals
RNA, Messenger
Cervix
Interleukin-6
Muscle, Smooth
Chlamydia Infections
biology.organism_classification
030104 developmental biology
Endocrinology
Gene Expression Regulation
Oxytocin
chemistry
Receptors, Prostaglandin E, EP4 Subtype
Subjects
Details
- ISSN :
- 15221555 and 01931849
- Volume :
- 318
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Endocrinology and Metabolism
- Accession number :
- edsair.doi.dedup.....8572e7dde16c37e458cbdb643b030afa