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Clostridium difficile Toxoid Vaccine Candidate Confers Broad Protection against a Range of Prevalent Circulating Strains in a Nonclinical Setting
- Source :
- Infection and Immunity
- Publication Year :
- 2018
- Publisher :
- American Society for Microbiology, 2018.
-
Abstract
- Clostridium difficile infection (CDI) is a leading cause of nosocomial and antibiotic-associated diarrhea. A vaccine, based on formalin-inactivated toxins A and B purified from anaerobic cultures of C. difficile strain VPI 10463 (toxinotype 0), has been in development for the prevention of symptomatic CDI. We evaluated the breadth of protection conferred by this C. difficile toxoid vaccine in cross-neutralization assessments using sera from vaccinated hamsters against a collection of 165 clinical isolates. Hamster antisera raised against the C. difficile toxoid vaccine neutralized the cytotoxic activity of culture supernatants from several toxinotype 0 strains and heterologous strains from 10 different toxinotypes. Further assessments performed with purified toxins confirmed that vaccine-elicited antibodies can neutralize both A and B toxins from a variety of toxinotypes. In the hamster challenge model, the vaccine conferred significant cross-protection against disease symptoms and death caused by heterologous C. difficile strains from the most common phylogenetic clades, including the most prevalent toxinotypes.
- Subjects :
- 0301 basic medicine
toxin-variant strains
efficacy
Bacterial Toxins
030106 microbiology
Immunology
Hamster
Heterologous
Biology
Microbiology
Enterotoxins
03 medical and health sciences
Bacterial Proteins
Cricetinae
Toxoid Vaccine
medicine
Animals
Cytotoxic T cell
Clostridium difficile toxoid vaccine
Antiserum
Mesocricetus
Clostridioides difficile
Bacterial Infections
Clostridium difficile
protection
Virology
Diarrhea
Infectious Diseases
Bacterial Vaccines
Clostridium Infections
biology.protein
Female
Parasitology
medicine.symptom
Antibody
Genome, Bacterial
Subjects
Details
- ISSN :
- 10985522 and 00199567
- Volume :
- 86
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity
- Accession number :
- edsair.doi.dedup.....8571ff97b0a6358d46dbccee27200ec0