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Activation of Postsynaptic Ca2+Stores Modulates Glutamate Receptor Cycling in Hippocampal Neurons
- Source :
- Journal of Neurophysiology. 93:178-188
- Publication Year :
- 2005
- Publisher :
- American Physiological Society, 2005.
-
Abstract
- We show that activation of postsynaptic inositol 1,4,5-tris-phosphate receptors (IP3Rs) with the IP3R agonist adenophostin A (AdA) produces large increases in AMPA receptor (AMPAR) excitatory postsynaptic current (EPSC) amplitudes at hippocampal CA1 synapses. Co-perfusion of the Ca2+chelator bis-( o-aminophenoxy)- N,N,N′,N′-tetraacetic acid strongly inhibited AdA-enhanced increases in EPSC amplitudes. We examined the role of AMPAR insertion/anchoring in basal synaptic transmission. Perfusion of an inhibitor of synaptotagmin-soluble n-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor SNARE-mediated exocytosis depressed basal EPSC amplitudes, whereas a peptide that inhibits GluR2/3 interactions with postsynaptic density-95 (PDZ) domain proteins glutamate receptor interacting protein (GRIP)/protein interacting with C-kinase-1 (PICK1) enhanced basal synaptic transmission. These results suggest that constitutive trafficking and anchoring of AMPARs help maintain basal synaptic transmission. The regulation of postsynaptic AMPAR trafficking involves synaptotagmin-SNARE-mediated vesicle exocytosis and interactions between AMPARs and the PDZ domains in GRIP/PICK1. We show that inhibitors of synaptotagmin-SNARE-mediated exocytosis, or interactions between AMPARs and GRIP/PICK1, attenuated AdA-enhanced increases in EPSC amplitudes. These results suggest that IP3R-mediated Ca2+release can enhance AMPAR EPSC amplitudes through mechanisms that involve AMPAR-PDZ interactions and/or synaptotagmin-SNARE-mediated receptor trafficking.
- Subjects :
- Agonist
Adenosine
Patch-Clamp Techniques
Physiology
medicine.drug_class
Vesicular Transport Proteins
Nerve Tissue Proteins
AMPA receptor
In Vitro Techniques
Hippocampal formation
Hippocampus
Synaptic Transmission
Rats, Sprague-Dawley
Synaptotagmins
chemistry.chemical_compound
Postsynaptic potential
Complement C2a
medicine
Animals
Drug Interactions
Inositol
Receptors, AMPA
Receptor
Egtazic Acid
Chelating Agents
Neurons
Membrane Glycoproteins
Chemistry
musculoskeletal, neural, and ocular physiology
General Neuroscience
Calcium-Binding Proteins
Glutamate receptor
Complement C2
Electric Stimulation
Rats
Calcium Channel Agonists
Animals, Newborn
Receptors, Glutamate
nervous system
Synapses
Excitatory postsynaptic potential
Calcium
Carrier Proteins
SNARE Proteins
Neuroscience
Subjects
Details
- ISSN :
- 15221598 and 00223077
- Volume :
- 93
- Database :
- OpenAIRE
- Journal :
- Journal of Neurophysiology
- Accession number :
- edsair.doi.dedup.....85685641ce04c55d8daba263d2e70f36
- Full Text :
- https://doi.org/10.1152/jn.00651.2004