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A TaqMan™-based quantitative PCR screening assay for the probiotic Streptococcus salivarius K12 based on the specific detection of its megaplasmid-associated salivaricin B locus
- Source :
- Journal of microbiological methods. 170
- Publication Year :
- 2019
-
Abstract
- In order to assess the colonization efficacy of the oral probiotic Streptococcus salivarius K12, a rapid method for specific detection and enumeration of the strain was developed. Here, we describe a two-step TaqMan™ quantitative PCR assay using primer-probe combinations targeting genes of the locus encoding the lantibiotic bacteriocin salivaricin B.
- Subjects :
- Microbiology (medical)
Specific detection
Locus (genetics)
Biology
Real-Time Polymerase Chain Reaction
Microbiology
Streptococcus salivarius
law.invention
03 medical and health sciences
Probiotic
Bacteriocin
Bacterial Proteins
law
TaqMan
Humans
Molecular Biology
Gene
030304 developmental biology
0303 health sciences
030306 microbiology
Probiotics
biology.organism_classification
Bacterial Load
Real-time polymerase chain reaction
bacteria
Plasmids
Subjects
Details
- ISSN :
- 18728359
- Volume :
- 170
- Database :
- OpenAIRE
- Journal :
- Journal of microbiological methods
- Accession number :
- edsair.doi.dedup.....85622e8a4ffac89d38b547da93a4bd71