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Oligosaccharides from Human Milk as Revealed by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
- Source :
- Analytical Biochemistry. 223:218-226
- Publication Year :
- 1994
- Publisher :
- Elsevier BV, 1994.
-
Abstract
- In this study neutral and acidic oligosaccharide fractions prepared from human milk have been investigated using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The fraction of neutral oligosaccharides was separated by gel permeation chromatography (GPC) and the resulting subfractions were analyzed by MALDI-MS using the positive ion mode. Several low-molecular-weight glycans (degree of polymerization up to 13) were observed whose structures have already been elucidated. In addition, a variety of so far unknown large-sized carbohydrates was detected whose molecular weights range from M(r) 2242 to 8000. The large-sized glycans which possess a low abundance appear to be composed of both lactosamine and fucose residues attached to the lactose unit at the reducing end of the sugar chains with a highly variable stochiometry. Following subfractionation by GPC, acidic (i.e., containing sialic acid) glycans were analyzed by MALDI-MS using both positive and negative ion mode. Because of the inferior stability of acidic glycans, various matrices were applied and compared with respect to signal intensity, resolution, and analyte stability.
- Subjects :
- Glycan
Resolution (mass spectrometry)
Biophysics
Oligosaccharides
Mass spectrometry
Sensitivity and Specificity
Biochemistry
Mass Spectrometry
Fucose
Gel permeation chromatography
chemistry.chemical_compound
Drug Stability
Polysaccharides
Humans
Molecular Biology
chemistry.chemical_classification
Chromatography
Milk, Human
Molecular Structure
biology
Molecular mass
Chemistry
Cell Biology
Oligosaccharide
Molecular Weight
Matrix-assisted laser desorption/ionization
Chromatography, Gel
Sialic Acids
biology.protein
Female
Subjects
Details
- ISSN :
- 00032697
- Volume :
- 223
- Database :
- OpenAIRE
- Journal :
- Analytical Biochemistry
- Accession number :
- edsair.doi.dedup.....85534850b7138847ae1320b8b3be51fc