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Sorghum Brown Midrib19 (Bmr19) Gene Links Lignin Biosynthesis to Folate Metabolism
- Source :
- Genes, Volume 12, Issue 5, Genes, Vol 12, Iss 660, p 660 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Genetic analysis of brown midrib sorghum (Sorghum bicolor) mutant lines assembled in our program has previously shown that the mutations fall into four allelic groups, bmr2, bmr6, bmr12 or bmr19. Causal genes for allelic groups bmr2, bmr6 and bmr12, have since been identified. In this report, we provide evidence for the nature of the bmr19 mutation. This was accomplished by introgressing each of the four bmr alleles into nine different genetic backgrounds. Polymorphisms from four resequenced bulks of sorghum introgression lines containing either mutation, relative to those of a resequenced bulk of the nine normal midrib recurrent parent lines, were used to locate their respective causal mutations. The analysis confirmed the previously reported causal mutations for bmr2 and bmr6 but failed in the case of bmr12-bulk due to a mixture of mutant alleles at the locus among members of that mutant bulk. In the bmr19-bulk, a common G → A mutation was found among all members in Sobic.001G535500. This gene encodes a putative folylpolyglutamate synthase with high homology to maize Bm4. The brown midrib phenotype co-segregated with this point mutation in two separate F2 populations. Furthermore, an additional variant allele at this locus obtained from a TILLING population also showed a brown midrib phenotype, confirming this locus as Bmr19.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Mutant
Population
lignin
Locus (genetics)
QH426-470
Biology
medicine.disease_cause
01 natural sciences
Genetic analysis
bmr19
03 medical and health sciences
lignocellulosic feedstock
Genetics
medicine
Allele
education
Genetics (clinical)
education.field_of_study
Mutation
Point mutation
Folylpolyglutamate synthase
brown midrib mutants
030104 developmental biology
sorghum
folylpolyglutamate synthase
010606 plant biology & botany
Subjects
Details
- ISSN :
- 20734425
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Genes
- Accession number :
- edsair.doi.dedup.....854f66351e95b19ddbc92f65cd0a6e43