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Effects of theabrownin on serum metabolites and gut microbiome in rats with a high-sugar diet
- Source :
- Foodfunction. 10(11)
- Publication Year :
- 2019
-
Abstract
- Evidence has proven that the gut microbiota is an important environmental factor contributing to obesity by altering host energy harvest and storage. We performed a high-throughput 16S rDNA sequencing association study and serum metabolomics profiling in rats with a high-sugar diet. Our studies revealed that the high sugar diet reduced the diversity of cecal microorganisms, while the combination of theabrownin and the high sugar diet increased the diversity of cecal microorganisms and promoted reproduction of Alloprevotella, Coprostanoligenes_group, Bacteroides, Prevotellaceae_NK3B31_group, Desulfovibrio, Intestinimonas, Alistipes, Bifidobacterium, Phascolarctobacterium, Ruminococcaceae_UCG-010 and Staphylococcus. The combination also inhibited the growth of Lactobacillus, Prevotellaceae_Ga6A1_group and Tyzzerella. The Firmicutes/Bacteroidetes (F/B) ratio can be significantly reduced after the intervention of theabrownin in high sugar diet rats, and the reproduction of Bacteroides acidifaciens (BA) and Staphylococcus saprophyticus subsp. saprophyticus can be promoted. We found that the obesity-associated gut microbial species were linked to the changes in circulating metabolites. Serum levels of deoxycholic acid, cholic acid, 1H-indole-3-acetic acid, 3-indole acrylic acid and melatonin were negatively correlated with BA and Staphylococcus saprophyticus subsp. saprophyticus, but positively correlated with Lactobacillus murinus, Leptum and Gut_metagenome. 2-Hydroxy-6-methylpyridin-3-carboxylic acid, l-homoserine, and 1,7-dimethylxanthine were positively correlated with BA and Staphylococcus saprophyticus subsp. saprophyticus, but negatively correlated with Lactobacillus murinus, Leptum, and Gut_metagenome. In a high sugar diet mode, theabrownin reduced the body weight and triglycerides and improved insulin resistance mainly by targeting the reproduction of intestinal microorganisms such as BA, Staphylococcus saprophyticus subsp. saprophyticus, Lactobacillus murinus, Leptum, Gut_metagenome and so on. A strong correlation between cecal microorganisms and serum metabolites, obesity and insulin resistance was observed. Theabrownin has high potential in reducing the risk of cardiovascular diseases such as diabetes and obesity.
- Subjects :
- 0301 basic medicine
Male
Firmicutes
Dietary Sugars
Cholic Acid
Gut flora
Prevotellaceae
Catechin
03 medical and health sciences
Lactobacillus
Animals
Humans
Food science
Obesity
Alistipes
Cecum
Bifidobacterium
Melatonin
Staphylococcus saprophyticus
030109 nutrition & dietetics
biology
Bacteria
Indoleacetic Acids
Body Weight
General Medicine
biology.organism_classification
Gastrointestinal Microbiome
Rats
030104 developmental biology
Bacteroides
Food Science
Subjects
Details
- ISSN :
- 2042650X
- Volume :
- 10
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Foodfunction
- Accession number :
- edsair.doi.dedup.....8543487607920f0d238160df824f30de