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Evaluation of the estrogenic activity of the wild Pueraria mirifica by vaginal cornification assay
- Source :
- The Journal of reproduction and development. 53(2)
- Publication Year :
- 2007
-
Abstract
- The aim of this study was to evaluate the estrogenic activity of tuberous samples of phytoestrogen-rich Pueraria mirifica collected from 25 of 76 provinces in Thailand by vaginal cornification assay. Tuberous powders were prepared and administered to ovariectomized rats for 14 consecutive days at dosages of 10, 100 and 1,000 mg/kg BW respectively, and were compared with a daily treatment with 2 mg/kg BW 17beta-estradiol (E(2)). Rats treated with 10 mg/kg BW Pueraria mirifica showed no vaginal cornification. Treatment with 100 mg/kg BW Pueraria mirifica from 13 out of 25 plant samples resulted in development of vaginal cornification. The cell count percentages of the vaginal smeared cells for the treatment with the 2 plant samples that exhibited the fastest vaginal cornification revealed large variation in their estrogenic activities. Treatment with 1,000 mg/kg BW Pueraria mirifica from all plant samples produced vaginal cornification with the mean value for the period (day) of first appearance of cornified cells being 4.08 days compared to 2 days with 2 mg/kg BW E(2). The overall appearance period (day) of cornified cells during the treatment and post-treatment period with 1,000 mg/kg BW per day Pueraria mirifica was shorter than treatment with 2 mg/kg BW E(2). The results demonstrate that the plant population shows differential estrogenic activity as evaluated by vaginal cornification assay.
- Subjects :
- medicine.medical_specialty
Dose
Ovariectomy
Phytoestrogens
Biology
Animal science
Species Specificity
Internal medicine
medicine
Animals
Rats, Wistar
Vaginal Smears
Mean value
Epithelial Cells
biology.organism_classification
Plant population
Rats
Plant Tubers
Pueraria
Endocrinology
Vagina
Ovariectomized rat
Animal Science and Zoology
Biological Assay
Female
Pueraria mirifica
Subjects
Details
- ISSN :
- 09168818
- Volume :
- 53
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- The Journal of reproduction and development
- Accession number :
- edsair.doi.dedup.....853f2550ed6f4be85c489dd68b1b78e4