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Low dose of methylmercury (MeHg) exposure induces caspase mediated-apoptosis in cultured neural progenitor cells
- Source :
- The Journal of toxicological sciences. 38(6)
- Publication Year :
- 2013
-
Abstract
- Methylmercury (MeHg) is an environmental pollutant known to cause neurobehavioral defects, and it is especially toxic to the developing brain. In contrast to the adult, the developing brain consists of a large number of dividing neural progenitor cells (NPCs), which are vulnerable targets for MeHg toxicity. In a previous study, we showed that the embryonic NPCs from the telencephalon are more sensitive to MeHg than other neural cells. Here, we investigated the mechanism of cell death underlying MeHg toxicity. We observed that exposure of NPCs to MeHg caused DNA laddering in a dose- and time-dependent manner. Decreased pro-caspase3 and increased cleaved-caspase3 protein was observed 3-12 hours after incubation of NPCs with MeHg. Moreover, the caspase-inhibitor Z-VAD FMK significantly suppressed MeHg-induced cell death in a dose-dependent manner. These results suggest that environmentally relevant levels of MeHg exposure induce apoptosis in NPCs.
- Subjects :
- Telencephalon
Programmed cell death
Time Factors
Apoptosis
DNA laddering
Toxicology
Amino Acid Chloromethyl Ketones
Mice
Neural Stem Cells
otorhinolaryngologic diseases
Animals
Caspase
Cells, Cultured
Mice, Inbred ICR
biology
Dose-Response Relationship, Drug
Chemistry
Caspase 3
DNA
Methylmercury Compounds
Embryonic stem cell
Caspase Inhibitors
Neural stem cell
Cell biology
stomatognathic diseases
Dose–response relationship
Toxicity
biology.protein
Environmental Pollutants
Subjects
Details
- ISSN :
- 18803989
- Volume :
- 38
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- The Journal of toxicological sciences
- Accession number :
- edsair.doi.dedup.....85388556b26a0e3cd4a789881ef2523c