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Calpains are involved in phosphatidylinositol 3',4'-bisphosphate synthesis dependent on the alpha IIb beta 3 integrin engagement in thrombin-stimulated platelets
- Source :
- FEBS letters. 404(1)
- Publication Year :
- 1997
-
Abstract
- In thrombin-stimulated platelets αIIbβ3 integrin engagement triggers both phosphatidylinositol 3′,4′-bisphosphate synthesis and calpain activation. We checked the possible involvement of calpains in phosphatidylinositol 3-kinase signalling pathway using a cell permeant specific inhibitor of calpains, calpeptin. In conditions where thrombin-induced platelet aggregation and secretion were not impaired, we found a dose-dependent inhibition of phosphatidylinositol 3,4-bisphosphate synthesis by calpeptin from 50 μg/ml. Moreover, pretreatment of platelets by both calpeptin and the peptide RGDS, an inhibitor of fibrinogen binding to activated αIIbβ3 integrin, did not induce additive effects on phosphatidylinositol 3,4-bisphosphate inhibition. Finally, the p85 regulatory subunit of phosphatidylinositol 3-kinase was still translocated to the cytoskeleton in calpeptin-treated platelets. These data indicate that calpains are involved in the regulation of αIIbβ3 integrin-dependent phosphatidylinositol 3-kinase signalling pathway.©1997 Federation of European Biochemical Societies.
- Subjects :
- Blood Platelets
Phosphatidylinositol 3,4-bisphosphate
Integrins
Integrin
Biophysics
Platelet Glycoprotein GPIIb-IIIa Complex
Phosphatidylinositol 3-Kinases
Biochemistry
Calpeptin
chemistry.chemical_compound
Thrombin
Phosphatidylinositol Phosphates
Structural Biology
Genetics
medicine
Humans
Platelet aggregation
Platelet
Phosphatidylinositol
Molecular Biology
Cytoskeleton
biology
Calpain
Fibrinogen binding
Biological Transport
Cell Biology
Dipeptides
Platelet Activation
αIIbβ3 integrin
Cell biology
Phosphotransferases (Alcohol Group Acceptor)
chemistry
biology.protein
Phosphatidylinositol 3-kinase
medicine.drug
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 404
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- FEBS letters
- Accession number :
- edsair.doi.dedup.....852b42c9f4b02ff2d2c180eb60dfa96b