Back to Search
Start Over
Promoter-associated endogenous and exogenous small RNAs suppress human bladder cancer cell metastasis by activating p21 CIP1/WAF1 expression
- Source :
- Tumor Biology. 37:6589-6598
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- Accumulating data suggest that micro RNAs (miRNAs) or double-stranded RNAs (dsRNAs) can activate gene expression by targeting promoters. The cyclin-dependent kinase inhibitor p21 (CIP1/WAF1) (p21) has also been shown to suppress epithelial-mesenchymal transition (EMT) which plays a crucial role in the early stage of tumor metastases and invasiveness. In a previous study, we have reported that miR-370-5p is low-expressed in bladder cancer (BCa) tissues and cell lines. Here, we identified that miR-370-5p and sequence homology dsRNA (dsP21-555) fully complementary to promoter hold the potent abilities to induce p21 expression. Moreover, transfection of miR-370-5p or dsP21-555 into BCa cells remarkably inverts EMT-associated genes (increases epithelial cell makers E-cadherin and β-catenin, and decreases mesenchymal cell markers ZEB1 and Vimentin) expression mainly via regulating p21 expression. Besides, through manipulating p21, both the candidates can retard BCa cell migration and invasion. In summary, our results provide evidence that both endogenous and exogenous small RNAs may function to induce p21 expression by interacting with the similar promoter region and impede BCa metastasis.
- Subjects :
- Cyclin-Dependent Kinase Inhibitor p21
Transcriptional Activation
0301 basic medicine
Epithelial-Mesenchymal Transition
RNA activation
Biology
03 medical and health sciences
0302 clinical medicine
Cell Movement
RNA interference
Cell Line, Tumor
Gene expression
microRNA
Humans
Epithelial–mesenchymal transition
Promoter Regions, Genetic
Cell Proliferation
RNA, Double-Stranded
Binding Sites
Base Sequence
Cell growth
General Medicine
Gene Expression Regulation, Neoplastic
MicroRNAs
RNA silencing
030104 developmental biology
Urinary Bladder Neoplasms
030220 oncology & carcinogenesis
Cancer cell
Cancer research
RNA Interference
Subjects
Details
- ISSN :
- 14230380 and 10104283
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Tumor Biology
- Accession number :
- edsair.doi.dedup.....851894de91234aaf6c34a6ebaf2d3042