Back to Search
Start Over
Demonstration of starter unit interprotein transfer from a fatty acid synthase to a multidomain, nonreducing polyketide synthase
- Source :
- Chembiochem : a European journal of chemical biology. 13(13)
- Publication Year :
- 2012
-
Abstract
- The pathway for substrate transacylation between a fungal type I fatty acid synthase (FAS) and a nonreducing polyketide synthase (NR-PKS) was determined by in vitro reconstitution of dissected domains. System kinetics were influenced by domain dissections, and the FAS phosphopantetheinyl transferase (PPT) monodomain exhibited coenzyme A selectivity for the post-translational activation of the FAS acyl carrier protein (ACP).
- Subjects :
- Stereochemistry
Coenzyme A
Acylation
Transferases (Other Substituted Phosphate Groups)
Saccharomyces cerevisiae
Biochemistry
Article
chemistry.chemical_compound
Transacylation
Protein structure
Bacterial Proteins
Polyketide synthase
Acyl Carrier Protein
Protein Interaction Maps
Molecular Biology
biology
Organic Chemistry
Fatty Acids
Substrate (chemistry)
Protein Structure, Tertiary
Acyl carrier protein
Fatty acid synthase
chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
biology.protein
Molecular Medicine
Fatty Acid Synthases
Polyketide Synthases
Subjects
Details
- ISSN :
- 14397633
- Volume :
- 13
- Issue :
- 13
- Database :
- OpenAIRE
- Journal :
- Chembiochem : a European journal of chemical biology
- Accession number :
- edsair.doi.dedup.....8516acdfb5c052af928369b82d7cfd4a