Back to Search
Start Over
Ikaros is critical for B cell differentiation and function
- Source :
- European journal of immunology. 32(3)
- Publication Year :
- 2002
-
Abstract
- The Ikaros gene encodes a zinc-finger transcription factor required during early B cell development, as B-lineage cells are absent in mice lacking Ikaros. Here we describe a novel Ikaros-targeted mouse line carrying a beta-galactosidase reporter in which low amounts of Ikaros proteins remain expressed. In homozygote animals, B cells are absent during fetal development, but develop postnatally from a reduced pool of precursors. In vitro, the proliferation and differentiation of B-lineage progenitors are severely impaired. These defects are attenuated in vivo, but bone marrow B cells display an unusual pattern of cell surface marker expression and show decreased transcript levels for TdT, Rag-1, Rag-2 and lambda 5. These abnormalities suggest a partial block at the proB cell stage of differentiation. In the periphery, mature B cells exhibit a lower activation threshold but form fewer germinal centers in response to antigenic stimulation. Our results show that Ikaros controls multiple aspects of B cell differentiation and function.
- Subjects :
- Genotype
Transcription, Genetic
Cellular differentiation
Genes, RAG-1
Immunology
Cell
Bone Marrow Cells
Mice, Transgenic
Biology
Ikaros Transcription Factor
Mice
Fetus
DNA Nucleotidylexotransferase
Genes, Reporter
medicine
Immunology and Allergy
Animals
Cell Lineage
Progenitor cell
Transcription factor
B cell
Homeodomain Proteins
B-Lymphocytes
Reverse Transcriptase Polymerase Chain Reaction
Immunologic Deficiency Syndromes
Gene targeting
Germinal center
Gene Expression Regulation, Developmental
Cell Differentiation
beta-Galactosidase
Molecular biology
Antigens, Differentiation, B-Lymphocyte
DNA-Binding Proteins
Immunoglobulin Isotypes
medicine.anatomical_structure
Phenotype
Immunoglobulin M
Lac Operon
Antibody Formation
Gene Targeting
Transcription Factors
Subjects
Details
- ISSN :
- 00142980
- Volume :
- 32
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- European journal of immunology
- Accession number :
- edsair.doi.dedup.....850e37f641d6354870968acd40be3bd1