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The Comparability of Anti-Spike SARS-CoV-2 Antibody Tests is Time-Dependent: a Prospective Observational Study
- Source :
- Microbiology spectrum. 10(1)
- Publication Year :
- 2022
-
Abstract
- ObjectivesVarious commercial anti-Spike SARS-CoV-2 antibody tests are used for studies and in clinical settings after vaccination. An international standard for SARS-CoV-2 antibodies has been established to achieve comparability of such tests, allowing conversions to BAU/ml. This study aimed to investigate the comparability of antibody tests regarding the timing of blood collection after vaccination.MethodsFor this prospective observational study, antibody levels of 50 participants with homologous AZD1222 vaccination were evaluated at 3 and 11 weeks after the first dose and 3 weeks after the second dose using two commercial anti-Spike binding antibody assays (Roche and Abbott) and a surrogate neutralization assay.ResultsThe correlation between Roche and Abbott changed significantly depending on the time point studied. Although 3 weeks after the first dose, Abbott provided values three times higher than Roche, 11 weeks after the first dose, the values for Roche were twice as high as for Abbott, and 3 weeks after the second dose even 5-6 times higher.ConclusionsThe comparability of quantitative anti-Spike SARS-CoV-2 antibody tests is highly dependent on the timing of blood collection after vaccination. Therefore, standardization of the timing of blood collection might be necessary for the comparability of different quantitative SARS-COV-2 antibody assays.Abstract Figure
- Subjects :
- Microbiology (medical)
Adult
medicine.medical_specialty
COVID-19 Vaccines
Time Factors
Physiology
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Antibody level
Antibodies, Viral
Gastroenterology
Internal medicine
medicine
Genetics
Humans
Prospective Studies
biology
General Immunology and Microbiology
Ecology
business.industry
SARS-CoV-2
Comparability
Vaccination
COVID-19
Blood collection
Cell Biology
Middle Aged
Antigen binding
Infectious Diseases
Spike Glycoprotein, Coronavirus
biology.protein
Observational study
Antibody
business
Subjects
Details
- ISSN :
- 21650497
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Microbiology spectrum
- Accession number :
- edsair.doi.dedup.....84c9a682fdbb10a3632bffa27e48285b