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Genomic analysis of a pneumovirus isolated from dogs with acute respiratory disease
- Source :
- Veterinary Microbiology. 150:88-95
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- A previously unrecognized virus belonging to the subfamily Pneumovirinae and most closely related to murine pneumovirus (MPV) was identified in domestic dogs in 2 related animal shelters. Additional diagnostic testing yielded 3 new viral isolates and identified 6 additional PCR positive dogs from other USA locations indicating that its distribution is not geographically limited. Nucleotide sequences encompassing 9 of the 10 genes were compared to the only 2 available MPV strains, 15 and J3666. Several features distinguished the canine pneumovirus (CnPnV) from the murine strains. Two regions of diversity were identified in the amino-proximal region of P and the overlapping P2 ORF was only 54 amino acids (aa) compared to 137aa in MPV. The G protein had an amino-terminal cytoplasmic tail 18aa longer than in the MPV strains. The CnPnV SH protein showed the highest divergence with only 90.2% aa identity when compared to MPV strain 15. Like strain 15, the CnPnV SH ORF coded for a protein of 92aa while J3666 has a 114aa variant. Comparison of CnPnV isolates at culture passages 4 and 17 revealed 7nt differences within the 8598nt sequenced. Of note was a substitution at nt 364 in G resulting in a termination codon that would produce a truncated G protein of 122aa. Analysis of early passage and ex vivo samples showed the termination codon in G to be predominant after 6 days in culture indicating rapid selection of the mutation in A72 cells.
- Subjects :
- Paramyxoviridae
Sequence analysis
Genome, Viral
Biology
Microbiology
Virus
Cell Line
Open Reading Frames
Viral Proteins
Pneumovirinae
Dogs
Animals
Pneumovirus Infections
Dog Diseases
Gene
Phylogeny
Pneumovirus
Sequence Homology, Amino Acid
General Veterinary
Sequence Analysis, RNA
Genomics
General Medicine
biology.organism_classification
Virology
United States
Stop codon
Open reading frame
RNA, Viral
Subjects
Details
- ISSN :
- 03781135
- Volume :
- 150
- Database :
- OpenAIRE
- Journal :
- Veterinary Microbiology
- Accession number :
- edsair.doi.dedup.....84bc98464744a8afffdccb76e1835474