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In search of the correlation between nanomechanical and biomolecular properties of prostate cancer cells with different metastatic potential
- Publication Year :
- 2021
-
Abstract
- Nanomechanical properties of living cells, as measured with atomic force microscopy (AFM), are increasingly recognized as criteria that differentiate normal and pathologically altered cells. Locally measured cell elastic properties, described by the parameter known as Young's modulus, are currently proposed as a new diagnostic parameter that can be used at the early stage of cancer detection. In this study, local mechanical properties of normal human prostate (RWPE-1) cells and a range of malignant (22Rv1) and metastatic prostate cells (LNCaP, Du145 and PC3) were investigated. It was found that non-malignant prostate cells are stiffer than cancer cells while the metastatic cells are much softer than malignant cells from the primary tumor site. Next, the biochemical properties of the cells were measured using confocal Raman (RS) and Fourier-transform infrared (FT-IR) spectroscopies to reveal these cells' biochemical composition as malignant transformation proceeds. Nanomechanical and biochemical profiles of five different prostate cell lines were subsequently analyzed using partial least squares regression (PLSR) in order to identify which spectral features of the RS and FT-IR spectra correlate with the cell's elastic properties. The PLSR-based model could predict Young's modulus values based on both RS and FT-IR spectral information. These outcomes show not only that AFM, RS and FT-IR techniques can be used for discrimination between normal and cancer cells, but also that a linear correlation between mechanical response and biomolecular composition of the cells that undergo malignant transformation can be found. This knowledge broadens our understanding of how prostate cancer cells evolve thorough the multistep process of tumor pathogenesis.
- Subjects :
- Male
0301 basic medicine
Confocal
Cell
Biophysics
Biochemistry
Malignant transformation
03 medical and health sciences
Prostate cancer
DU145
Cell Line, Tumor
LNCaP
medicine
Humans
Neoplasm Metastasis
Molecular Biology
Mechanical Phenomena
030102 biochemistry & molecular biology
Chemistry
Prostatic Neoplasms
medicine.disease
Primary tumor
Biomechanical Phenomena
030104 developmental biology
medicine.anatomical_structure
Cancer cell
Cancer research
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....84b63d8fb1da649bda2df7880afc3dfd