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Expression of DNA Methyltransferases Is Influenced by Growth Hormone in the Long-Living Ames Dwarf Mouse In Vivo and In Vitro
- Source :
- The Journals of Gerontology Series A: Biological Sciences and Medical Sciences. 69:923-933
- Publication Year :
- 2013
- Publisher :
- Oxford University Press (OUP), 2013.
-
Abstract
- Methyltransferase expression and DNA methylation are linked to aging and age-related disease. We utilized 3-, 12-, and 24-month-old Ames dwarf and their wild-type siblings to examine the genotype and age-related differences in the expression of methyltransferase enzymes related to DNA methylation in the liver, glycine-N-methyltransferase and DNA methyltransferase (DNMT). We found that DNMT proteins and transcripts are differentially expressed in dwarf mice compared with wild-type siblings that can be attributed to age and/or genotype. However, DNMT1 protein expression is drastically reduced compared with wild-type controls at every age. DNMT3a protein levels coincide with differences observed in DNMT activity. Growth hormone appears to modulate expression of DNMT1 and 3a in dwarf liver tissue and primary hepatocytes. Therefore, growth hormone may contribute to age-related processes, DNA methylation, and, ultimately, longevity.
- Subjects :
- Aging
Methyltransferase
Immunoblotting
Longevity
Repressor
Dwarfism
Mice, Inbred Strains
Glycine N-Methyltransferase
Biology
DNA methyltransferase
DNA Methyltransferase 3A
Mice
chemistry.chemical_compound
Genotype
medicine
Animals
DNA (Cytosine-5-)-Methyltransferases
Dwarfism, Pituitary
Methyltransferases
DNA Methylation
medicine.disease
Molecular biology
Repressor Proteins
chemistry
Growth Hormone
DNA methylation
Hepatocytes
DNMT1
Original Article
Colorimetry
Geriatrics and Gerontology
DNA
Subjects
Details
- ISSN :
- 1758535X and 10795006
- Volume :
- 69
- Database :
- OpenAIRE
- Journal :
- The Journals of Gerontology Series A: Biological Sciences and Medical Sciences
- Accession number :
- edsair.doi.dedup.....84a98704dfa04535d1c9eb0886a74e48