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Sea Cucumber Lipid-Soluble Extra Fraction Prevents Ovalbumin-Induced Allergic Airway Inflammation
- Source :
- Journal of Medicinal Food. 21:21-29
- Publication Year :
- 2018
- Publisher :
- Mary Ann Liebert Inc, 2018.
-
Abstract
- In a previous study, our research group demonstrated that sea cucumber (Apostichopus japonicus) extracts ameliorated allergic airway inflammation through CD4+CD25+Foxp3+ T (regulatory T; Treg) cell activation and recruitment to the lung. In this study, we aimed to determine which components of sea cucumber contribute to the amelioration of airway inflammation. We used n-hexane fractionation to separate sea cucumber into three phases (n-hexane, alcohol, and solid) and evaluated the ability of each phase to elevate Il10 expression in splenocytes and ameliorate symptoms in mice with ovalbumin (OVA)/alum-induced asthma. Splenocytes treated with the n-hexane phase showed a significant increase in Il10 expression. In the n-hexane phase, 47 fatty acids were identified. Individual fatty acids that comprised at least 5% of the total fatty acids were 16:0, 16:1n-7, 18:0, 18:1n-7, 20:4n-6, and 20:5n-3 (eicosapentaenoic acid). After administering the n-hexane phase to mice with OVA/alum-induced asthma, their asthma symptoms were ameliorated. Several immunomodulatory effects were observed in the n-hexane phase-pretreated group, compared with a vehicle control group. First, eosinophil infiltration and goblet cell hyperplasia were significantly reduced around the airways. Second, the concentrations of Th2-related cytokines (IL-4, IL-5, and IL-13) and Th17-related cytokines (IL-17) were significantly decreased in the spleen and bronchoalveolar lavage fluid (BALF). Finally, the concentrations of TGF-β and IL-10, which are associated with Treg cells, were significantly increased in the BALF and splenocyte culture medium. In conclusion, a fatty acid-rich fraction (n-hexane phase) of sea cucumber extract ameliorated allergic airway inflammation in a mouse model.
- Subjects :
- 0301 basic medicine
Ovalbumin
Sea Cucumbers
Anti-Inflammatory Agents
Medicine (miscellaneous)
Biology
Pharmacology
Mice
03 medical and health sciences
Th2 Cells
0302 clinical medicine
medicine
Splenocyte
Animals
Humans
Lung
chemistry.chemical_classification
Nutrition and Dietetics
medicine.diagnostic_test
Fatty Acids
Fatty acid
respiratory system
Eosinophil
biology.organism_classification
Eicosapentaenoic acid
Asthma
Mice, Inbred C57BL
030104 developmental biology
Bronchoalveolar lavage
medicine.anatomical_structure
chemistry
Immunology
Apostichopus japonicus
biology.protein
Cytokines
Th17 Cells
Female
030211 gastroenterology & hepatology
Cell activation
Bronchoalveolar Lavage Fluid
Subjects
Details
- ISSN :
- 15577600 and 1096620X
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Journal of Medicinal Food
- Accession number :
- edsair.doi.dedup.....84a2be2a8565df3a67a73cde26e70470