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Marker-assisted elimination of drought-susceptible accessions in upland rice breeding
- Source :
- Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), instacron:EMBRAPA
- Publication Year :
- 2018
-
Abstract
- Breeding for water-deficit tolerance is fundamental to guarantee the sustainability of upland rice production, mainly due to the possibility of an increased frequency of drought episodes due to climate change. This work aimed to identify single nucleotide polymorphism (SNP) markers, derived from RNA sequencing (RNA-Seq), genome-wide association study (GWAS) and candidate genes from Arabidopsis, with potential for use in marker-assisted selection (MAS) for drought tolerance. RNA-Seq and GWAS were efficient in identifying useful SNP markers from the data obtained from three years of field experiments for 175 upland rice accessions, which were sequenced using 32 genes by Capture-Seq. Three genes were equally able to generate SNP markers that discriminated 95% of the 20 most drought susceptible accessions in the joint analysis of the experiments. The elimination of the genotypes with the unfavourable SNP allele reduced the initial number of accessions to one third, and transferring this result in a breeding routine, would enable to conduct smaller experiments per target location, increasing the precision and reducing the cost of the drought phenotyping. Made available in DSpace on 2018-03-10T00:34:10Z (GMT). No. of bitstreams: 1 CNPAF2018gmr.pdf: 333924 bytes, checksum: 202e7a3c0bca406bccdf93cad82d91f8 (MD5) Previous issue date: 2018-03-08
- Subjects :
- 0301 basic medicine
Candidate gene
Genetic techniques and protocols
Drought tolerance
Melhoramento genético vegetal
Genome-wide association study
Single-nucleotide polymorphism
Biology
Upland rice
Genotyping by sequencing
Plant breeding
03 medical and health sciences
Genotype
Genetics
SNP
Allele
Molecular Biology
Resistência a seca
Nucleotide sequences
business.industry
Sequence analysis
food and beverages
General Medicine
Biotechnology
Single nucleotide polymorphism
030104 developmental biology
Arroz
Rice
business
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), instacron:EMBRAPA
- Accession number :
- edsair.doi.dedup.....84a185c897473124c6489cbedfabdd67