Back to Search
Start Over
Hydrophilicity nano-titania coating modified magnetic graphene oxide for pass-through cleanup of fipronil and its metabolites in human blood
- Source :
- Journal of chromatography. A. 1553
- Publication Year :
- 2017
-
Abstract
- A novel hydrophilicity nano-titania coating modified magnetic graphene oxide (HTC-Mag-GO) has been synthesized. It has been evaluated in PRiME (process, robustness, improvements, matrix effects, ease of use) pass-through cleanup procedure for human blood prior to analysis of fipronil and its metabolites, i.e., fipronil sulphone, fipronil sulphide and fipronil desulfinyl by liquid chromatography-tandem quadrupole mass spectrometry (LC–MS/MS). Compared with the Oasis PRiME HLB cartridge, HTC-Mag-GO is much more effective for the removal of matrix effect. Furthermore, it is beneficial to protect the chromatographic column and ESI source by the HTC-Mag-GO PRiME pass-through cleanup procedure, resulting from the much cleaner blood samples. Under the optimum conditions, the results show higher cleanup efficiency of HTC-Mag-GO with recoveries in the range of 92.4%–108%. Especially, the HTC-Mag-GO is also evaluated for reuse (20 times) without much sacrifice of the cleanup efficiency. The limits of quantification (LOQs) for fipronil, fipronil sulphone, fipronil sulphide and fipronil desulfinyl are 8.9 ng/L, 7.2 ng/L, 8.0 ng/L and 42 ng/L, respectively. The developed method has also been successfully applied to monitoring fipronil and its metabolites in 120 blood samples, and fipronil sulphone is detected in six samples with concentrations in the range of 12.1 ng/L–106 ng/L. Further, the well validation results and the application to analysis of fipronil and its metabolites in real samples demonstrate the applicability to toxico kinetic studies and clinical studies.
- Subjects :
- Oxide
010501 environmental sciences
engineering.material
Mass spectrometry
01 natural sciences
Biochemistry
Analytical Chemistry
law.invention
chemistry.chemical_compound
Coating
law
Tandem Mass Spectrometry
Nano titania
Humans
Fipronil sulphone
Fipronil
0105 earth and related environmental sciences
Titanium
Chromatography
Human blood
Graphene
Magnetic Phenomena
010401 analytical chemistry
Organic Chemistry
Oxides
General Medicine
0104 chemical sciences
Kinetics
chemistry
engineering
Nanoparticles
Pyrazoles
Graphite
Hydrophobic and Hydrophilic Interactions
Chromatography, Liquid
Subjects
Details
- ISSN :
- 18733778
- Volume :
- 1553
- Database :
- OpenAIRE
- Journal :
- Journal of chromatography. A
- Accession number :
- edsair.doi.dedup.....848fc88e1537467d26e8ff1cbd4797d4