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Estrogen deficiency stimulates B lymphopoiesis in mouse bone marrow
- Source :
- The Journal of clinical investigation. 94(3)
- Publication Year :
- 1994
-
Abstract
- We have found that an estrogen deficiency causes a marked increase in bone marrow cells. To examine the effect of estrogen on hemopoiesis, we characterized the increased population of bone marrow cells after ovariectomy (OVX). In OVX mice, the percentage of myeloid cells and granulocytes was decreased, whereas that of B220-positive B lymphocytes was selectively increased 2-4 wk after surgery. The total number of myeloid cells and granulocytes did not change appreciably, but that of B220-positive cells was greatly increased by OVX. When OVX mice were treated with estrogen, the increased B lymphopoiesis returned to normal. B220-positive cells were classified into two subpopulations, B220I0W and B220i9'h. The majority of the B220'W cells were negative for the IgM ,u chain, whereas most of the B220"'4" cells were ,i-positive. OVX selectively increased the precursors of B lymphocytes identified by B2201w. ,unegative phenotype, suggesting that an estrogen deficiency stimulates accumulation ofB lymphocyte precursors. When bone marrow-derived stromal cells (ST2) were pretreated with estrogen then co-cultured with bone marrow cells in the presence of estrogen, the stromal cell-dependent B lymphopoiesis was greatly inhibited. The present study suggests that estrogen plays an important role in the regulation ofB lymphocyte development in mouse bone marrow. (J. Clin. Invest. 1994. 94:1090-1097.) Key words: ovariectomy * hemopoiesis * B lymphocytes estrogen * stromal cells
- Subjects :
- medicine.medical_specialty
Stromal cell
Time Factors
medicine.drug_class
Lymphocyte
Cellular differentiation
Ovariectomy
Population
Fluorescent Antibody Technique
Bone Marrow Cells
Mice, Inbred Strains
Biology
Cell Line
Mice
Bone Marrow
Reference Values
Internal medicine
medicine
Animals
education
Cells, Cultured
education.field_of_study
B-Lymphocytes
Estradiol
Immunoglobulin mu-Chains
Cell Differentiation
General Medicine
Hematopoietic Stem Cells
Haematopoiesis
medicine.anatomical_structure
Endocrinology
Immunoglobulin M
Estrogen
Cell culture
Delayed-Action Preparations
Female
Bone marrow
hormones, hormone substitutes, and hormone antagonists
Cell Division
Research Article
Subjects
Details
- ISSN :
- 00219738
- Volume :
- 94
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- The Journal of clinical investigation
- Accession number :
- edsair.doi.dedup.....8478c2d5e3d76df439869dbaba741d9d