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Effects of Tetramethylpyrazine on Neuronal Apoptosis in the Superficial Dorsal Horn in a Rat Model of Neuropathic Pain
- Source :
- The American Journal of Chinese Medicine. 40:1229-1239
- Publication Year :
- 2012
- Publisher :
- World Scientific Pub Co Pte Ltd, 2012.
-
Abstract
- The Bennett and Xie (1988) model of chronic constriction injury (CCI) investigated the effects of tetramethylpyrazine (TMP) on neuropathic pain-associated behaviors and neuronal apoptosis in the spinal dorsal horn. Fifty-four male rats were randomly divided into sham (group S), CCI (group C) and TMP groups (group T). Each group was divided into subgroups (n = 6 in each group) according the time of sacrifice: 3 d, 7 d and 14 d. Rat sciatic nerves were unligated (group S), or the right sciatic nerve was loosely ligated (groups C and T) to produce CCI. Mechanical withdrawal thresholds (MWTs) and thermal withdrawal latencies (TWLs) were measured, and the rats were sacrificed at different time points post-operation. The L4-L6 sections of the spinal cord were removed. Apoptotic changes were evaluated using the TUNEL method. Immunohistochemistry assessed Bcl-2 and caspase-3 expression. TMP treatment increased MWT and TWL values and Bcl-2 expression, but it reduced neuronal apoptosis and caspase-3 expression in laminae I–II of the spinal dorsal horn. These results suggested that the inhibition of neuronal apoptosis via the modulation of Bcl-2 and caspase-3 proteins in the rat spinal dorsal horn contributed to TMP-induced analgesia.
- Subjects :
- Male
medicine.medical_specialty
Apoptosis
Caspase 3
chemistry.chemical_compound
Internal medicine
In Situ Nick-End Labeling
medicine
Animals
Tetramethylpyrazine
Neurons
TUNEL assay
Horn (anatomy)
business.industry
General Medicine
Spinal cord
Immunohistochemistry
Rats
Posterior Horn Cells
Disease Models, Animal
medicine.anatomical_structure
Endocrinology
Complementary and alternative medicine
chemistry
Pyrazines
Anesthesia
Neuropathic pain
Neuralgia
business
Subjects
Details
- ISSN :
- 17936853 and 0192415X
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- The American Journal of Chinese Medicine
- Accession number :
- edsair.doi.dedup.....8476f6f0070212d42aa78362ddc35bf3