Construction of Synthetic Antibody Libraries

Authors :: Vincent Parez
Déborah Caucheteur
Gautier Robin
Pierre Martineau
Institut de Recherche en Cancérologie de Montpellier (IRCM - U1194 Inserm - UM)
CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)
Source :: Antibody Engineering ISBN: 9781493986477, Antibody Engineering, Antibody Engineering, 1827, pp.93-108, 2018, Methods in Molecular Biology, 978-1-4939-8647-7. ⟨10.1007/978-1-4939-8648-4_5⟩
Publication Year :: 2018
Publisher :: Springer New York, 2018.
Abstract: International audience; Libraries of antibody fragments displayed on filamentous phages are now a widely used approach to isolate antibodies against virtually any target. We describe a simple protocol to make large and diverse libraries based on a single or a limited number of frameworks. The approach is flexible enough to be used with any antibody format, either single-chain (scFv, VHH) or multi-chain (Fv, Fab, (Fab')2), and to target in a single step the six complementarity-determining regions-or any other part-of the antibody molecule. Using this protocol, libraries larger than 1010 can be constructed in a single week.

ISBN :: 978-1-4939-8647-7
ISBNs :: 9781493986477
Database :: OpenAIRE
Journal :: Antibody Engineering ISBN: 9781493986477, Antibody Engineering, Antibody Engineering, 1827, pp.93-108, 2018, Methods in Molecular Biology, 978-1-4939-8647-7. ⟨10.1007/978-1-4939-8648-4_5⟩
Accession number :: edsair.doi.dedup.....84635d00617663afa9deacc132e5e9de

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