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Identification and characterization of a pathogenicity-related gene VdCYP1 from Verticillium dahliae
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Verticillium dahliae is a phytopathogenic fungus that causes vascular wilt disease in a wide variety of crop plants, thereby causing extensive economic loss. In present study, one V. dahliae T-DNA mutant M01C06 showed the pathogenicity loss on cotton, and the expression of a flanking gene encoding cytochrome P450 monooxygenase (P450, VdCYP1) was strongly repressed. P450s of fungi could affect the fungal pathogenicity by involving in the synthesis of secondary metabolites. However, there was no report about the pathogenic function of P450s in V. dahliae. VdCYP1 gene deletion and complementation experiments confirmed that VdCYP1 was the pathogenicity-related gene in V. dahliae. A comparison of culture supernatants of the VdCYP1 deletion mutants and wild-type strains indicates that at least 14 kinds of secondary metabolites syntheses were affected due to VdCYP1 gene deletion. One of these compounds, sulfacetamide, had the ability to induce the necrosis and wilting symptoms in cotton. Above results indicate that VdCYP1 could participate in pathogenesis by involving the secondary metabolism in V. dahliae, such as the compound sulfacetamide. In conclusion, VdCYP1 acts as an important pathogenicity-related factor to involve in secondary metabolism that likely contributes to the pathogenic process in V. dahliae.
- Subjects :
- DNA, Bacterial
0106 biological sciences
0301 basic medicine
Mutant
Virulence
Verticillium
01 natural sciences
Article
Microbiology
03 medical and health sciences
Cytochrome P-450 Enzyme System
Sulfacetamide
Gene Expression Regulation, Fungal
Verticillium dahliae
Secondary metabolism
Plant Diseases
Wilt disease
Regulation of gene expression
Gossypium
Multidisciplinary
biology
Fungal genetics
Spores, Fungal
biology.organism_classification
DNA-Binding Proteins
Complementation
030104 developmental biology
Transcription Factors
010606 plant biology & botany
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....845681a6f6d1578a07f4dc8d72e1e8b0