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An approach to rapid distributed manufacturing of broad spectrum anti-viral griffithsin using cell-free systems to mitigate pandemics
- Source :
- bioRxiv
- Publication Year :
- 2023
- Publisher :
- Elsevier BV, 2023.
-
Abstract
- This study describes the cell-free biomanufacturing of a broad-spectrum antiviral protein, griffithsin (GRFT) such that it can be produced with consistent purity and potency in less than 24 hours. We demonstrate GRFT production using two independent cell-free systems, one plant and one microbial. Griffithsin purity and quality were verified using standard regulatory metrics. Efficacy was demonstratedin vitroagainst SARS-CoV-2 and HIV-1 and was nearly identical to that of GRFT expressedin vivo. The proposed production process is efficient and can be readily scaled up and deployed anywhere in the world where a viral pathogen might emerge. The current emergence of viral variants has resulted in frequent updating of existing vaccines and loss of efficacy for front-line monoclonal antibody therapies. Proteins such as GRFT with its efficacious and broad virus neutralizing capability provide a compelling pandemic mitigation strategy to promptly suppress viral emergence at the source of an outbreak.
- Subjects :
- Bioengineering
General Medicine
Molecular Biology
Article
Biotechnology
Subjects
Details
- ISSN :
- 18716784
- Volume :
- 76
- Database :
- OpenAIRE
- Journal :
- New Biotechnology
- Accession number :
- edsair.doi.dedup.....84542effbec6bea138fdb5f6cf3364cb