Back to Search
Start Over
Evaluation of in vitro penetration of fluticasone propionate from MP-AzeFlu and fluticasone propionate nasal spray through EpiAirway (TM) 606 tissues using vertical diffusion cells
- Source :
- JOURNAL OF ASTHMA AND ALLERGY, Journal of Asthma and Allergy
- Publication Year :
- 2020
-
Abstract
- Purpose: Most patients with allergic rhinitis (AR) have moderate-to-severe disease, requiring complete and prompt relief when symptoms occur. The time course of fluticasone propionate (FP) penetration into nasal tissues after intranasal administration is not well characterized. The goal of this proof-of-concept study was to evaluate the mucosal penetration of FP from fixed-combination FP-azelastine nasal spray (MP-AzeFlu) compared with an FP-only nasal spray in an in vitro, 3-dimensional human bronchial tissue model. Materials and Methods: Absorption of FP from MP-AzeFlu and FP nasal spray was modeled using EpiAirway (TM) 606 (MatTek Corporation; Ashland, MA, USA) tissue cultured in vertical diffusion cells. The dosing amount of MP-AzeFlu was optimized in a pilot study. Based on the results of the pilot study, 10 mu L of MP-AzeFlu (3.65 mu g; n = 8) and 10 mu L of FP nasal spray (5.00 mu g; n = 8) were evaluated for penetration of tissue. Tissue integrity was monitored with Lucifer yellow. FP in the receiving media was quantified for each sample using liquid chromatography with tandem mass spectrometry. Results: MP-AzeFlu and FP nasal spray were associated with similar FP accumulation profiles in the receiving media, but the permeability of FP was greater for MP-AzeFlu during hours 0 to 6, suggesting faster absorption for MP-AzeFlu. No indications of compromised tissue integrity were found in any of the tested cells. Conclusion: The higher and more rapid penetration of FP from MP-AzeFlu supports the use of MP-AzeFlu for patients with AR, particularly when prioritizing fast and pronounced symptom relief.
- Subjects :
- Pulmonary and Respiratory Medicine
mucosal penetration
BIOAVAILABILITY
medicine.medical_treatment
Pharmacology
Fluticasone propionate
03 medical and health sciences
0302 clinical medicine
Symptom relief
Bronchial tissue
Medicine and Health Sciences
Immunology and Allergy
Medicine
0601 history and archaeology
AZELASTINE
Original Research
allergic rhinitis
060102 archaeology
business.industry
06 humanities and the arts
Penetration (firestop)
MP29-02
In vitro
INTRANASAL THERAPY
ALLERGIC RHINITIS
030228 respiratory system
Nasal spray
azelastine hydrochloride
Time course
Nasal administration
pharmacology
business
absorption
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 11786965
- Database :
- OpenAIRE
- Journal :
- JOURNAL OF ASTHMA AND ALLERGY, Journal of Asthma and Allergy
- Accession number :
- edsair.doi.dedup.....83f74c8b3e2999d8bc2597a00435b143