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Protein molecule stratification inside a single curved film: Evidence from X-ray scattering
- Source :
- Soft Matter, Soft Matter, Royal Society of Chemistry, 2011, 7 (19), pp.9283. ⟨10.1039/C1SM06010D⟩, Soft Matter, Royal Society of Chemistry, 2011, 7 (19), pp.9283. ⟨10.1039/c1sm06010d⟩, Soft Matter, 2011, 7 (19), pp.9283. ⟨10.1039/c1sm06010d⟩
- Publication Year :
- 2011
- Publisher :
- HAL CCSD, 2011.
-
Abstract
- International audience; We have studied single curved films stabilized by globular proteins, using small angle scattering. By combining both the use of in-house X-ray and synchrotron radiation, we have measured the structural properties of films (thickness, electronic density) by controlling the physicochemical properties of protein (ovalbumin, pH 7, bulk concentration 10 g L−1). For each experiment, solutions of highly purified protein were freshly prepared to eliminate any problem of aging. The observation of Kiessig fringes shows that the films are thin with an average thickness of 60 nm. Benefiting from the fine angular resolution and the short acquisition time of a synchrotron source, we have highlighted a stratification formation inside the films. This phenomenon suggests protein structural reorganization under confinement, possibly driven by high osmotic pressure.
- Subjects :
- Materials science
Globular protein
[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]
Analytical chemistry
Synchrotron radiation
02 engineering and technology
010402 general chemistry
01 natural sciences
law.invention
rayon-X
law
Molecule
chemistry.chemical_classification
molécule
propriété physico-chimique
Scattering
X-ray
Protéine
General Chemistry
021001 nanoscience & nanotechnology
Condensed Matter Physics
Synchrotron
0104 chemical sciences
chemistry
Chemical physics
pression osmotique
Small-angle scattering
0210 nano-technology
Electronic density
Subjects
Details
- Language :
- English
- ISSN :
- 1744683X and 17446848
- Database :
- OpenAIRE
- Journal :
- Soft Matter, Soft Matter, Royal Society of Chemistry, 2011, 7 (19), pp.9283. ⟨10.1039/C1SM06010D⟩, Soft Matter, Royal Society of Chemistry, 2011, 7 (19), pp.9283. ⟨10.1039/c1sm06010d⟩, Soft Matter, 2011, 7 (19), pp.9283. ⟨10.1039/c1sm06010d⟩
- Accession number :
- edsair.doi.dedup.....83f484d4533f5684e341cbc4c1321e82