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Comparison of anticoagulant effects on vein grafts between human TFPI gene transfection and aspirin oral administration
- Source :
- Journal of Huazhong University of Science and Technology [Medical Sciences]. 28:147-151
- Publication Year :
- 2008
- Publisher :
- Springer Science and Business Media LLC, 2008.
-
Abstract
- To develop a more efficient antithrombotic way after coronary artery bypass grafting (CABG), the anticoagulant effects were compared of human tissue factor pathway inhibitor (TFPI) gene transfection and aspirin oral administration (traditional method) on vein grafts. An eukaryotic expression plasmid pCMV-(Kozak) TFPI was prepared. Animal model of carotid artery bypass grafting was constructed. In operation, endothelial cells of vein grafts in TFPI group and empty plasmid control group were transfected with pCMV-(Kozak) TFPI and empty plasmid pCMV respectively, while no transfection was conducted in aspirin control group. After operation, aspirin (2 mg.kg(-1).(-1)) was administered (i.g.) in aspirin control group. Three days later, grafts (n=10) were harvested for RT-PCR, Western blotting and immunohistochemical analyses of exogenous gene expression and for pathological, scanning electron microscopic observation of thrombus. Thirty days later, the patency rates of remnant grafts (n=10) were recorded by vessel Doppler ultrasonography. Human TFPI gene products were detected in gene transferred vein grafts. Three days later, thrombi were found in 7 animals of aspirin control group and in 8 animals of empty plasmid control group, but in only 1 of TFPI group (P0.01). Thirty days later, 5 grafts were occluded in empty plasmid control group, but none of grafts was occluded in the other groups (P0.05). The endothelial surfaces of grafts in both of the control groups were covered with aggregated erythrocytes and platelets, and it were not seen in TFPI group. It was suggested that the anticoagulant effects on vein grafts of human TFPI gene transfection are better than those of aspirin.
- Subjects :
- medicine.medical_specialty
medicine.drug_class
Lipoproteins
Biomedical Engineering
Administration, Oral
Pharmacology
Transfection
Biochemistry
Veins
Biomaterials
Tissue factor pathway inhibitor
Oral administration
Genetics
medicine
Animals
Humans
Platelet
Coronary Artery Bypass
Thrombus
Earth-Surface Processes
Venous Thrombosis
Aspirin
business.industry
Anticoagulant
Anticoagulants
Ultrasonography, Doppler
medicine.disease
Surgery
Disease Models, Animal
Venous thrombosis
Tissue Transplantation
Rabbits
business
Plasmids
medicine.drug
Subjects
Details
- ISSN :
- 19931352 and 16720733
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Journal of Huazhong University of Science and Technology [Medical Sciences]
- Accession number :
- edsair.doi.dedup.....83ed8f1ab0e272a766d76ec6cd081ba4